Abstract：
:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatment suppressed both phaseolin bands seen in untreated or control cells, and resulted in a single species (M r 43 kDa). We provide evidence that the observed size heterogeneity arises by asymmetric glycosylation of a single, high-molecular weight precursor. These results also indicate that differential glycosylation of phaseolin polypeptides can occur on the product of a single gene, and, in that sense, is not dependent on amino acid sequence variations. Phaseolin accumulates to a very high level (90 µg/10(6) cells), 90% of it being secreted into the culture medium. Immuno-gold staining and electron microscopy demonstrated phaseolin polypeptides in electron-dense, membrane-bound vesicles seen at the periphery of the cytoplasm of infect cells and in cytoplasmic multivesicular bodies. The effect on protein accumulation of a single-basepair transversion (G»C) at position +6 is also described. This study constitutes, to our knowledge, one of the first instances of a plant protein being expressed in insect cells and suggests possible differences in the sorting mechanisms of glycoproteins from legume seeds and those from Spodoptera frugiperda cell line Sf9.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TCdoi
10.1007/BF00033603subject
Has Abstractpub_date
1988-11-01 00:00:00pages
475-88issue
6eissn
0167-4412issn
1573-5028journal_volume
10pub_type
杂志文章abstract：KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01045-4
更新日期：2020-10-01 00:00:00
abstract：:Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-008-9374-9
更新日期：2009-03-01 00:00:00
abstract：:Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0445-z
更新日期：2016-05-01 00:00:00
abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040674
更新日期：1992-02-01 00:00:00
abstract：:The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023992
更新日期：1991-03-01 00:00:00
abstract：:Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014876409166
更新日期：2002-03-01 00:00:00
abstract：:Alternatively spliced introns are the ones that are usually spliced but can be occasionally retained in a transcript isoform. They are the most frequently used alternative splice form in plants (~50% of alternative splicing events). Chlamydomonas reinhardtii, a unicellular alga, is a good model to understand alternati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0605-9
更新日期：2017-06-01 00:00:00
abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017779
更新日期：1984-09-01 00:00:00
abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020326
更新日期：1986-11-01 00:00:00
abstract：:We describe the molecular and functional characterization of three closely related S-adenosyl-L-methionine synthetase (SAMS) isoenzymes from Catharanthus roseus (Madagascar periwinkle). The genes are differentially expressed in cell cultures during growth of the culture and after application of various stresses (elici...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005711720930
更新日期：1997-01-01 00:00:00
abstract：:Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice m...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00911-0
更新日期：2019-11-01 00:00:00
abstract：:A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0161-x
更新日期：2014-04-01 00:00:00
abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019126
更新日期：1995-10-01 00:00:00
abstract：:Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9100-4
更新日期：2007-03-01 00:00:00
abstract：:Darjeeling teas are the highest grown teas in the world and preferred for its flavour, aroma and quality. Apart from the genetic makeup of the plant, earlier reports suggest that insect infestation, particularly jassids and thrips triggers the aroma and flavour formation in Darjeeling tea. The present work encompasses...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9887-0
更新日期：2012-04-01 00:00:00
abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005708611020
更新日期：1997-04-01 00:00:00
abstract：:The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015643
更新日期：1987-03-01 00:00:00
abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00166458
更新日期：1987-05-01 00:00:00
abstract：:Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-1004-1
更新日期：2005-11-01 00:00:00
abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0531-2
更新日期：2016-12-01 00:00:00
abstract：:The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005874404706
更新日期：1997-08-01 00:00:00
abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0403-1
更新日期：2016-01-01 00:00:00
abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020626
更新日期：1985-09-01 00:00:00
abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005909703600
更新日期：1998-05-01 00:00:00
abstract：:Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9017-y
更新日期：2006-09-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01064-1
更新日期：2020-12-01 00:00:00
abstract：:The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019303
更新日期：1993-11-01 00:00:00
abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006030007333
更新日期：1998-08-01 00:00:00
abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1021266320877
更新日期：2002-12-01 00:00:00
