当前位置： SCI文献检索 > PLANT MOLECULAR BIOLOGY期刊下所有文献 > Protein-protein interactions in the secretory pathway, a growing demand for experimental approaches in vivo.

Protein-protein interactions in the secretory pathway, a growing demand for experimental approaches in vivo.

Abstract：

:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transport assays opens up the implementation of in vivo approaches which can be used to verify models based on in vitro work. The purpose of this review is to provide an overview of the various approaches involving living cells to resolve interactions between proteins that control complex mechanisms. In particular, it is illustrated how combinations of several methods can establish whether postulated interactions are of biological relevance or due to artefacts inherent to the experimental set-up.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Pimpl P,Denecke J

doi

10.1023/a:1021266320877

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

887-902

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

50

pub_type

杂志文章,评审

相关文献

PLANT MOLECULAR BIOLOGY文献大全
  • Developmental regulation of the PsbS gene expression in spinach seedlings: the role of phytochrome.

    abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00019467

    authors： Adamska I,Funk C,Renger G,Andersson B

    更新日期：1996-07-01 00:00:00

  • Genetic linkage facilitates cloning of Ert-m regulating plant architecture in barley and identified a strong candidate of Ant1 involved in anthocyanin biosynthesis.

    abstract：:The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-015-0350-x

    authors： Zakhrabekova S,Dockter C,Ahmann K,Braumann I,Gough SP,Wendt T,Lundqvist U,Mascher M,Stein N,Hansson M

    更新日期：2015-08-01 00:00:00

  • Characterization of a novel cis-acting element that is responsive to a fungal elicitor in the promoter of a tobacco class I chitinase gene.

    abstract：:The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00024116

    authors： Fukuda Y,Shinshi H

    更新日期：1994-02-01 00:00:00

  • Characterization of rps17, rp19 and rpl15: three nucleus-encoded plastid ribosomal protein genes.

    abstract：:Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00019207

    authors： Thompson MD,Jacks CM,Lenvik TR,Gantt JS

    更新日期：1992-03-01 00:00:00

  • Lysine-rich modified gamma-zeins accumulate in protein bodies of transiently transformed maize endosperms.

    abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005889314967

    authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

    更新日期：1997-05-01 00:00:00

  • Comparison of the expression of two highly homologous members of the soybean ribulose-1,5-bisphosphate carboxylase small subunit gene family.

    abstract：:Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00019389

    authors： Shirley BW,Ham DP,Senecoff JF,Berry-Lowe SL,Zurfluh LL,Shah DM,Meagher RB

    更新日期：1990-06-01 00:00:00

  • Cyclophilins are encoded by a small gene family in rice.

    abstract：:cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00028878

    authors： Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

    更新日期：1994-08-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.). 8. Cis-elements and trans-acting factors for the quantitative expression of a bean chalcone synthase gene promoter in electroporated alfalfa protoplasts.

    abstract：:A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00015079

    authors： Harrison MJ,Choudhary AD,Dubery I,Lamb CJ,Dixon RA

    更新日期：1991-05-01 00:00:00

  • Phenotypic changes in Arabidopsis caused by expression of a yeast vacuolar Ca2+/H+ antiporter.

    abstract：:In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1010620227913

    authors： Hirschi KD,Miranda ML,Wilganowski NL

    更新日期：2001-05-01 00:00:00

  • Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense.

    abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1021162321527

    authors： Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

    更新日期：2003-01-01 00:00:00

  • Structure and expression of the barley lipid transfer protein gene Ltp1.

    abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00040674

    authors： Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

    更新日期：1992-02-01 00:00:00

  • Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco.

    abstract：:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00027070

    authors： Lawton K,Ward E,Payne G,Moyer M,Ryals J

    更新日期：1992-08-01 00:00:00

  • Seed-specific increased expression of 2S albumin promoter of sesame qualifies it as a useful genetic tool for fatty acid metabolic engineering and related transgenic intervention in sesame and other oil seed crops.

    abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-014-0233-6

    authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

    更新日期：2014-11-01 00:00:00

  • Molecular characterization of the gene coding for GPRP, a class of proteins rich in glycine and proline interacting with membranes in Arabidopsis thaliana.

    abstract：:The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00049336

    authors： Marty I,Monfort A,Stiefel V,Ludevid D,Delseny M,Puigdomènech P

    更新日期：1996-02-01 00:00:00

  • A homeodomain leucine zipper gene from Craterostigma plantagineum regulates abscisic acid responsive gene expression and physiological responses.

    abstract：:A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-006-0023-x

    authors： Deng X,Phillips J,Bräutigam A,Engström P,Johannesson H,Ouwerkerk PB,Ruberti I,Salinas J,Vera P,Iannacone R,Meijer AH,Bartels D

    更新日期：2006-06-01 00:00:00

  • Synthesis versus degradation: directions of amino acid metabolism during Arabidopsis abiotic stress response.

    abstract：KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-018-0767-0

    authors： Hildebrandt TM

    更新日期：2018-09-01 00:00:00

  • Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco.

    abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00042040

    authors： Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

    更新日期：1995-04-01 00:00:00

  • Fractionation and characterization of polyadenylated RNA from broad bean meristematic root cells.

    abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00023013

    authors： Trapy G,Favre A,Esnault R

    更新日期：1981-03-01 00:00:00

  • Induction of chilling resistance by water stress, and cDNA sequence analysis and expression of water stress-regulated genes in rice.

    abstract：:Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00039544

    authors： Takahashi R,Joshee N,Kitagawa Y

    更新日期：1994-10-01 00:00:00

  • Evidence implying only unprimed RdRP activity during transitive gene silencing in plants.

    abstract：:RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-005-7307-4

    authors： Petersen BO,Albrechtsen M

    更新日期：2005-07-01 00:00:00

  • An improved chemically inducible gene switch that functions in the monocotyledonous plant sugar cane.

    abstract：:Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-013-0140-2

    authors： Kinkema M,Geijskes RJ,Shand K,Coleman HD,De Lucca PC,Palupe A,Harrison MD,Jepson I,Dale JL,Sainz MB

    更新日期：2014-03-01 00:00:00

  • Oryza sativa heat-induced RING finger protein 1 (OsHIRP1) positively regulates plant response to heat stress.

    abstract：KEY MESSAGE:OsHIRP1 is an E3 ligase that acts as a positive regulator in the plant response to heat stress, thus providing important information relating to adaptation and regulation under heat stress in plant. Extreme temperature adversely affects plant growth, development, and productivity. Here, we report the molecu...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-019-00835-9

    authors： Kim JH,Lim SD,Jang CS

    更新日期：2019-04-01 00:00:00

  • Studies on the effects of a flanking repetitive sequence on the expression of single-copy transgenes in Nicotiana sylvestris and in N. sylvestris-N. tomentosiformis hybrids.

    abstract：:To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sy...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1023937006311

    authors： Kunz C,Narangajavana J,Jakowitsch J,Park YD,Delon TR,Kovarik A,Koukalová B,van der Winden J,Moscone E,Aufsatz W,Mette MF,Matzke M,Matzke AJ

    更新日期：2003-05-01 00:00:00

  • Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response.

    abstract：:We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00042073

    authors： Fahrendorf T,Ni W,Shorrosh BS,Dixon RA

    更新日期：1995-08-01 00:00:00

  • Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class.

    abstract：:We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00028970

    authors： Roche D,Temple SJ,Sengupta-Gopalan C

    更新日期：1993-09-01 00:00:00

  • A view about the function of auxin-binding proteins at plasma membranes.

    abstract：:The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...

    journal_title：Plant molecular biology

    pub_type： 杂志文章,评审

    doi：10.1007/BF00019401

    authors： Klämbt D

    更新日期：1990-06-01 00:00:00

  • Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding.

    abstract：:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00020093

    authors： Helentjaris T,King G,Slocum M,Siedenstrang C,Wegman S

    更新日期：1985-03-01 00:00:00

  • Construction of a Chlamydomonas reinhardtii mutant with an intronless psbA gene.

    abstract：:Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00038998

    authors： Johanningmeier U,Heiss S

    更新日期：1993-04-01 00:00:00

  • The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis.

    abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-005-4922-z

    authors： Zhang L,Tao J,Wang S,Chong K,Wang T

    更新日期：2006-03-01 00:00:00

  • Insect resistance of transgenic plants that express modified Bacillus thuringiensis cryIA(b) and cryIC genes: a resistance management strategy.

    abstract：:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00039519

    authors： van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

    更新日期：1994-10-01 00:00:00