Two classes of differentially regulated glutamine synthetase genes are expressed in the soybean nodule: a nodule-specific class and a constitutively expressed class.

Abstract:

:We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the other set is expressed in other tissues, besides the nodules. The nodule-specific GS genes are expressed in a developmentally regulated manner in the nodules, independent of the onset of nitrogen fixation. The other class of GS genes is expressed constitutively in all tissues tested, but its expression level is dramatically enhanced in nodules following onset of N2 fixation. The latter set of genes is also expressed in cotyledons of germinating seedlings in a developmentally regulated manner. Analysis of hybrid select translation products and genomic Southern blots suggests that multiple gene members in each class are expressed in the nodules.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Roche D,Temple SJ,Sengupta-Gopalan C

doi

10.1007/BF00028970

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

971-83

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

22

pub_type

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