Abstract：
:The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 TATA box-beta-glucuronidase (GUS) reporter gene construct and transformed into tobacco. In all transgenic lines aerobic expression values were in the range obtained for negative controls while histochemical GUS assays reveal some weak expression in roots only. Anaerobic induction of about 100-fold to more than 1000-fold above unspecific background is mediated by a region of about 190 bp of the GapC4 promoter. Anaerobic reporter gene induction strongly decreases upon deletion of a 20 bp fragment from -286 to -266 relative to the transcription start point. This fragment harbours putative cis-acting sequences. Electrophoretic mobility shift assays with a 50 bp fragment harbouring these cis sequences reveal a high-mobility complex that is formed with nuclear extracts from aerobic and anaerobic leaf tissue while an additional low-mobility complex is anaerobiosis-specific. The formation of the high-mobility complex requires the sequence GTGGGCCCG. The 50 bp fragment alone confers weak and orientation-dependent anaerobic induction to a GapC4 TATA box-beta-glucuronidase (GUS) reporter gene.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Geffers R,Cerff R,Hehl Rdoi
10.1023/a:1006419232075subject
Has Abstractpub_date
2000-05-01 00:00:00pages
11-21issue
1eissn
0167-4412issn
1573-5028journal_volume
43pub_type
杂志文章abstract：:A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020982
更新日期：1995-11-01 00:00:00
abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006126609534
更新日期：1999-02-01 00:00:00
abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9428-z
更新日期：2009-02-01 00:00:00
abstract：:Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015971
更新日期：1993-06-01 00:00:00
abstract：:The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006146627301
更新日期：1999-01-01 00:00:00
abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0397-8
更新日期：2016-01-01 00:00:00
abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042240
更新日期：1996-06-01 00:00:00
abstract：:The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2141-7
更新日期：2004-08-01 00:00:00
abstract：:The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010665831955
更新日期：2001-05-01 00:00:00
abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006178710443
更新日期：1999-01-01 00:00:00
abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-6268-6
更新日期：2006-05-01 00:00:00
abstract：:Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006199003756
更新日期：1999-01-01 00:00:00
abstract：:Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0546-8
更新日期：2017-01-01 00:00:00
abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006282507095
更新日期：1999-07-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：:To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010614522706
更新日期：2001-07-01 00:00:00
abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000019067.05185.d6
更新日期：2003-11-01 00:00:00
abstract：:Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006118304093
更新日期：1999-04-01 00:00:00
abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0233-6
更新日期：2014-11-01 00:00:00
abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01037-4
更新日期：2020-09-01 00:00:00
abstract：:Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017721
更新日期：1990-07-01 00:00:00
abstract：:The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027380
更新日期：1993-08-01 00:00:00
abstract：:Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9784-y
更新日期：2011-07-01 00:00:00
abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0195-8
更新日期：2014-07-01 00:00:00
abstract：:Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans found in all higher plants. Although the precise function(s) of any single AGP is unknown, they are implicated in diverse developmental roles such as differentiation, cell-cell recognition, embryogenesis and programmed cell death. DNA sequencing pro...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019121
更新日期：1995-10-01 00:00:00
abstract：:The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021797
更新日期：1996-05-01 00:00:00
abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9722-4
更新日期：2011-02-01 00:00:00
abstract：:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023427
更新日期：1991-04-01 00:00:00
abstract：:We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006038117130
更新日期：1998-12-01 00:00:00
