PCNA protein associates to Cdk-A type protein kinases in germinating maize.

abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

journal_title：Plant molecular biology

authors： Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

更新日期：2016-01-01 00:00:00

abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

journal_title：Plant molecular biology

authors： Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

更新日期：2011-05-01 00:00:00

abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

journal_title：Plant molecular biology

authors： Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

更新日期：2002-10-01 00:00:00

abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...

journal_title：Plant molecular biology

authors： McNay JW,Pring DR,Lonsdale DM

更新日期：1983-07-01 00:00:00

abstract：:Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...

journal_title：Plant molecular biology

authors： Joshi CP,Nguyen HT

更新日期：1996-06-01 00:00:00

abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

journal_title：Plant molecular biology

authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

更新日期：2009-02-01 00:00:00

abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...

journal_title：Plant molecular biology

authors： Breton C,Chaboud A,Matthys-Rochon E,Bates EE,Cock JM,Fromm H,Dumas C

更新日期：1995-01-01 00:00:00

abstract：:More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...

journal_title：Plant molecular biology

authors： Perlick AM,Pühler A

更新日期：1993-09-01 00:00:00

abstract：:The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...

journal_title：Plant molecular biology

authors： Kuroda H,Sugiura M

更新日期：2014-12-01 00:00:00

abstract：:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

journal_title：Plant molecular biology

authors： Harms K,Wöhner RV,Schulz B,Frommer WB

更新日期：1994-11-01 00:00:00

abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

journal_title：Plant molecular biology

authors： Ringli C,Keller B

更新日期：1998-08-01 00:00:00

abstract：:The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

journal_title：Plant molecular biology

authors： Geffers R,Cerff R,Hehl R

更新日期：2000-05-01 00:00:00

abstract：:Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sit...

journal_title：Plant molecular biology

authors： Uemukai K,Iwakawa H,Kosugi S,de Uemukai S,Kato K,Kondorosi E,Murray JA,Ito M,Shinmyo A,Sekine M

更新日期：2005-01-01 00:00:00

abstract：:The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...

journal_title：Plant molecular biology

authors： Pilgrim ML,Caspar T,Quail PH,McClung CR

更新日期：1993-10-01 00:00:00

abstract：KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...

journal_title：Plant molecular biology

authors： Kant R,Dasgupta I

更新日期：2019-05-01 00:00:00

abstract：:Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...

journal_title：Plant molecular biology

authors： Wang H,Guo Y,Lv F,Zhu H,Wu S,Jiang Y,Li F,Zhou B,Guo W,Zhang T

更新日期：2010-03-01 00:00:00

abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...

journal_title：Plant molecular biology

authors： Kohno-Murase J,Murase M,Ichikawa H,Imamura J

更新日期：1994-11-01 00:00:00

abstract：:Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...

journal_title：Plant molecular biology

authors： Doğramaci M,Horvath DP,Chao WS,Foley ME,Christoffers MJ,Anderson JV

更新日期：2010-05-01 00:00:00

abstract：:A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...

journal_title：Plant molecular biology

authors： Leal I,Misra S

更新日期：1993-02-01 00:00:00

abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

journal_title：Plant molecular biology

authors： Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

更新日期：1999-01-01 00:00:00

abstract：:Electrophoretic analysis of translation products of polyadenylated RNA isolated from mid-maturation sorghum seed in the presence of [(35)S]met, [(3)H]leu, or [(3)H]val revealed two major proteins of kDa and 21 kDa. These products were not detected when [(3)H]lys was supplied as the radioactive substrate. Under similar...

journal_title：Plant molecular biology

authors： Derose RT,Ma DP,Kwon IS,Hasnain SE,Klassy RC,Hall TC

更新日期：1989-03-01 00:00:00

abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

journal_title：Plant molecular biology

authors： Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

更新日期：2019-08-01 00:00:00

abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

journal_title：Plant molecular biology

authors： Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

更新日期：2020-12-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

journal_title：Plant molecular biology

authors： Romano CP,Robson PR,Smith H,Estelle M,Klee H

更新日期：1995-03-01 00:00:00

abstract：:Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...

journal_title：Plant molecular biology

authors： Jongsma M,Koornneef M,Zabel P,Hille J

更新日期：1987-09-01 00:00:00

abstract：:The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...

journal_title：Plant molecular biology

authors： Mirabella R,Martirani L,Lamberti A,Iaccarino M,Chiurazzi M

更新日期：1999-01-01 00:00:00

abstract：:Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...

journal_title：Plant molecular biology

authors： Griffor MC,Vodkin LO,Singh RJ,Hymowitz T

更新日期：1991-07-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...

journal_title：Plant molecular biology

authors： Nanjo Y,Maruyama K,Yasue H,Yamaguchi-Shinozaki K,Shinozaki K,Komatsu S

更新日期：2011-09-01 00:00:00