Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.

abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...

journal_title：Plant molecular biology

authors： Gepstein S,Glick BR

更新日期：2013-08-01 00:00:00

abstract：:The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...

journal_title：Plant molecular biology

authors： Vincentz M,Leite A,Neshich G,Vriend G,Mattar C,Barros L,Weinberg D,de Almeida ER,de Carvalho MP,Aragão F,Gander ES

更新日期：1997-08-01 00:00:00

abstract：:Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...

journal_title：Plant molecular biology

authors： Mohamed A,Jansson C

更新日期：1991-05-01 00:00:00

abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

journal_title：Plant molecular biology

authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

更新日期：1992-01-01 00:00:00

abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

journal_title：Plant molecular biology

authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

更新日期：2001-11-01 00:00:00

abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

journal_title：Plant molecular biology

authors： Ringli C,Keller B

更新日期：1998-08-01 00:00:00

abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...

journal_title：Plant molecular biology

authors： Jensen MK,Rung JH,Gregersen PL,Gjetting T,Fuglsang AT,Hansen M,Joehnk N,Lyngkjaer MF,Collinge DB

更新日期：2007-09-01 00:00:00

abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

journal_title：Plant molecular biology

authors： Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

更新日期：2005-02-01 00:00:00

abstract：:A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...

journal_title：Plant molecular biology

authors： Goldsbrough A,Bevan M

更新日期：1991-02-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

journal_title：Plant molecular biology

authors： Susi P,Hohkuri M,Wahlroos T,Kilby NJ

更新日期：2004-01-01 00:00:00

abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

journal_title：Plant molecular biology

authors： Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

更新日期：1994-01-01 00:00:00

abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

journal_title：Plant molecular biology

authors： Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

更新日期：2017-04-01 00:00:00

abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

journal_title：Plant molecular biology

authors： Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

更新日期：1998-02-01 00:00:00

abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

journal_title：Plant molecular biology

authors： Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

更新日期：1999-01-01 00:00:00

abstract：:WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...

journal_title：Plant molecular biology

authors： Zheng L,Liu G,Meng X,Liu Y,Ji X,Li Y,Nie X,Wang Y

更新日期：2013-07-01 00:00:00

abstract：:The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by anoth...

journal_title：Plant molecular biology

authors： Mikkonen A,Porali I,Cercos M,Ho TH

更新日期：1996-05-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：:The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

journal_title：Plant molecular biology

authors： Nolan RC,Lin LS,Ho TH

更新日期：1987-01-01 00:00:00

abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...

journal_title：Plant molecular biology

authors： Müller BS,Sakamoto T,de Menezes IP,Prado GS,Martins WS,Brondani C,de Barros EG,Vianello RP

更新日期：2014-11-01 00:00:00

abstract：:Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...

journal_title：Plant molecular biology

authors： Fukuda N,Ikawa Y,Aoyagi T,Kozaki A

更新日期：2013-07-01 00:00:00

abstract：KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...

journal_title：Plant molecular biology

authors： Gong P,Luo Y,Huang F,Chen Y,Zhao C,Wu X,Li K,Yang X,Cheng F,Xiang X,Wu C,Pan G

更新日期：2019-05-01 00:00:00

abstract：KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...

journal_title：Plant molecular biology

authors： Chu P,Wilson GM,Michael TP,Vaiciunas J,Honig J,Lam E

更新日期：2018-10-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...

journal_title：Plant molecular biology

authors： Richard C,Ouellet H,Guertin M

更新日期：2000-01-01 00:00:00

abstract：:We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...

journal_title：Plant molecular biology

authors： Rohrmeier T,Lehle L

更新日期：1993-08-01 00:00:00

abstract：:The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...

journal_title：Plant molecular biology

authors： Lindstrom JT,Chu B,Belanger FC

更新日期：1993-12-01 00:00:00

abstract：:Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...

journal_title：Plant molecular biology

authors： Studart-Guimarães C,Gibon Y,Frankel N,Wood CC,Zanor MI,Fernie AR,Carrari F

更新日期：2005-11-01 00:00:00

abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...

journal_title：Plant molecular biology

authors： Xiang C,Miao ZH,Lam E

更新日期：1996-11-01 00:00:00

abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

journal_title：Plant molecular biology

authors： Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

更新日期：2002-10-01 00:00:00