Calmodulin-like proteins from Arabidopsis and tomato are involved in host defense against Pseudomonas syringae pv. tomato.

Abstract:

:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM) detect Ca(2+) signals and regulate downstream targets as part of a coordinated cellular response to a given stimulus. Here we report the characterization of a tomato gene (APR134) encoding a CaM-related protein that is induced in disease-resistant leaves in response to attack by Pseudomonas syringae pv. tomato. We show that suppression of APR134 gene expression in tomato (Solanum lycopersicum), using virus-induced gene silencing (VIGS), compromises the plant's immune response. We isolated APR134-like genes from Arabidopsis, termed CML42 and CML43, to investigate whether they serve a functionally similar role. Gene expression analysis revealed that CML43 is rapidly induced in disease-resistant Arabidopsis leaves following inoculation with Pseudomonas syringae pv. tomato. Overexpression of CML43 in Arabidopsis accelerated the hypersensitive response. Recombinant APR134, CML42, and CML43 proteins all bind Ca(2+ )in vitro. Collectively, our data support a role for CML43, and APR134 as important mediators of Ca(2+)-dependent signals during the plant immune response to bacterial pathogens.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

doi

10.1007/s11103-005-8395-x

subject

Has Abstract

pub_date

2005-08-01 00:00:00

pages

887-897

issue

6

eissn

0167-4412

issn

1573-5028

pii

10.1007/s11103-005-8395-x

journal_volume

58

pub_type

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