Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein.

Abstract:

:The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein from maize and Arabidopsis ERabp1. The deduced amino acid sequence contains an endoplasmic reticulum retention signal, the KDEL sequence located at the C-terminal end, and has two possible auxin-binding sites, HRHSCE and YDDWSVPHTA conserved sequences. Northern hybridization analysis revealed that the Ca-ERabp1 gene is differentially expressed in total RNA isolated from different organs of a pepper plant, showing the highest level of expression in fruits but barely detectable in leaves and roots.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Choi SY

doi

10.1007/BF00020496

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

995-7

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

32

pub_type

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