Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2.

Abstract:

:Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specific to low CO2-adapting cells. Six classes of low CO2-inducible clones were identified. One class of clone, reported here, represents a novel gene associated with adaptation of cells to air. A second class of clones corresponds to the air-inducible periplasmic carbonic anhydrase I (CAH1). These clones represent genes that respond to the level of CO2 in the environment.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Burow MD,Chen ZY,Mouton TM,Moroney JV

doi

10.1007/BF00021807

subject

Has Abstract

pub_date

1996-05-01 00:00:00

pages

443-8

issue

2

eissn

0167-4412

issn

1573-5028

journal_volume

31

pub_type

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