Abstract:
:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco that closely resembled hypersensitive response lesions. Mutational analysis identified the C-terminal-half peptide and the same amino acids indispensable for both enzyme and elicitor activity. His-SsCut was caused cell death in Arabidopsis, soybean (Glycine max), oilseed rape (Brassica napus), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum), indicating that both dicot and monocot species are responsive to the elicitor. Furthermore, the elicitation of tobacco was effective in the induction of the activities of hydrogen peroxide, phenylalanine ammonia-lyase, peroxides, and polyphenol oxidase. His-SsCut-treated plants exhibited enhanced resistance as indicated by a significant reduction in the number and size of S. sclerotiorum, Phytophthora sojae, and P. nicotianae lesions on leaves relative to controls. Real-time PCR results indicated that the expression of defense-related genes and genes involved in signal transduction were induced by His-SsCut. Our results demonstrate that SsCut is an elicitor that triggers defense responses in plants and will help to clarify its relationship to downstream signaling pathways that induce defense responses.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Zdoi
10.1007/s11103-014-0244-3subject
Has Abstractpub_date
2014-11-01 00:00:00pages
495-511issue
4-5eissn
0167-4412issn
1573-5028journal_volume
86pub_type
杂志文章abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036968
更新日期:1989-05-01 00:00:00
abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021193
更新日期:1995-07-01 00:00:00
abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016047231597
更新日期:2002-09-01 00:00:00
abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040644
更新日期:1991-09-01 00:00:00
abstract::Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9779-8
更新日期:2011-05-01 00:00:00
abstract::Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9816-7
更新日期:2011-11-01 00:00:00
abstract::More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028969
更新日期:1993-09-01 00:00:00
abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005780529457
更新日期:1997-01-01 00:00:00
abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/B:PLAN.0000028797.63168.a7
更新日期:2004-01-01 00:00:00
abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418241
更新日期:1985-07-01 00:00:00
abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006146627301
更新日期:1999-01-01 00:00:00
abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1015500431421
更新日期:2002-08-01 00:00:00
abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9428-z
更新日期:2009-02-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9448-8
更新日期:2009-04-01 00:00:00
abstract::The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0465-8
更新日期:2016-06-01 00:00:00
abstract::Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0546-8
更新日期:2017-01-01 00:00:00
abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016019
更新日期:1989-12-01 00:00:00
abstract::The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005867215258
更新日期:1997-05-01 00:00:00
abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005959203396
更新日期:1997-12-01 00:00:00
abstract::Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9926-x
更新日期:2012-07-01 00:00:00
abstract::The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040578
更新日期:1994-01-01 00:00:00
abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028878
更新日期:1994-08-01 00:00:00
abstract::This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006056128129
更新日期:1998-12-01 00:00:00
abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019864222515
更新日期:2002-10-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005790927581
更新日期:1997-03-01 00:00:00
abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027342
更新日期:1992-05-01 00:00:00