A novel protein elicitor (SsCut) from Sclerotinia sclerotiorum induces multiple defense responses in plants.

Abstract:

:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco that closely resembled hypersensitive response lesions. Mutational analysis identified the C-terminal-half peptide and the same amino acids indispensable for both enzyme and elicitor activity. His-SsCut was caused cell death in Arabidopsis, soybean (Glycine max), oilseed rape (Brassica napus), rice (Oryza sativa), maize (Zea mays), and wheat (Triticum aestivum), indicating that both dicot and monocot species are responsive to the elicitor. Furthermore, the elicitation of tobacco was effective in the induction of the activities of hydrogen peroxide, phenylalanine ammonia-lyase, peroxides, and polyphenol oxidase. His-SsCut-treated plants exhibited enhanced resistance as indicated by a significant reduction in the number and size of S. sclerotiorum, Phytophthora sojae, and P. nicotianae lesions on leaves relative to controls. Real-time PCR results indicated that the expression of defense-related genes and genes involved in signal transduction were induced by His-SsCut. Our results demonstrate that SsCut is an elicitor that triggers defense responses in plants and will help to clarify its relationship to downstream signaling pathways that induce defense responses.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Zhang H,Wu Q,Cao S,Zhao T,Chen L,Zhuang P,Zhou X,Gao Z

doi

10.1007/s11103-014-0244-3

subject

Has Abstract

pub_date

2014-11-01 00:00:00

pages

495-511

issue

4-5

eissn

0167-4412

issn

1573-5028

journal_volume

86

pub_type

杂志文章
  • Regulation of chalcone flavanone isomerase (CHI) gene expression inPetunia hybrida: the use of alternative promoters in corolla, anthers and pollen.

    abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00036968

    authors: van Tunen AJ,Hartman SA,Mur LA,Mol JN

    更新日期:1989-05-01 00:00:00

  • Molecular cloning of two tandemly arranged peroxidase genes from Populus kitakamiensis and their differential regulation in the stem.

    abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021193

    authors: Osakabe K,Koyama H,Kawai S,Katayama Y,Morohoshi N

    更新日期:1995-07-01 00:00:00

  • Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana.

    abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1016047231597

    authors: Osakabe K,Yoshioka T,Ichikawa H,Toki S

    更新日期:2002-09-01 00:00:00

  • The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin.

    abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040644

    authors: Spence MJ,Henzl MT,Lammers PJ

    更新日期:1991-09-01 00:00:00

  • DORNRÖSCHEN-LIKE expression marks Arabidopsis floral organ founder cells and precedes auxin response maxima.

    abstract::Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9779-8

    authors: Chandler JW,Jacobs B,Cole M,Comelli P,Werr W

    更新日期:2011-05-01 00:00:00

  • Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells.

    abstract::Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9816-7

    authors: Majerová E,Fojtová M,Mozgová I,Bittová M,Fajkus J

    更新日期:2011-11-01 00:00:00

  • A survey of transcripts expressed specifically in root nodules of broadbean (Vicia faba L.).

    abstract::More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028969

    authors: Perlick AM,Pühler A

    更新日期:1993-09-01 00:00:00

  • Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element.

    abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005780529457

    authors: Grimmig B,Matern U

    更新日期:1997-01-01 00:00:00

  • Characteristics of RNA silencing in plants: similarities and differences across kingdoms.

    abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/B:PLAN.0000028797.63168.a7

    authors: Susi P,Hohkuri M,Wahlroos T,Kilby NJ

    更新日期:2004-01-01 00:00:00

  • Quantitative variation in components of the maize mitochondrial genome between tissues and between plants with different male-sterile cytoplasms.

    abstract::The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF02418241

    authors: Abbott AG,O'Dell M,Flavell RB

    更新日期:1985-07-01 00:00:00

  • Conservation of rice genetic resources: the role of the International Rice Genebank at IRRI.

    abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:

    authors: Jackson MT

    更新日期:1997-09-01 00:00:00

  • The soybean ENOD40(2) promoter is active in Arabidopsis thaliana and is temporally and spatially regulated.

    abstract::The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006146627301

    authors: Mirabella R,Martirani L,Lamberti A,Iaccarino M,Chiurazzi M

    更新日期:1999-01-01 00:00:00

  • The DNA-compacting protein DCP68 from soybean chloroplasts is ferredoxin:sulfite reductase and co-localizes with the organellar nucleoid.

    abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1015500431421

    authors: Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

    更新日期:2002-08-01 00:00:00

  • Identification and characterization of a hypoallergenic ortholog of Ara h 2.01.

    abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9428-z

    authors: Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

    更新日期:2009-02-01 00:00:00

  • Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.

    abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-0393-x

    authors: Ogita S,Uefuji H,Morimoto M,Sano H

    更新日期:2004-04-01 00:00:00

  • FLEXIBLE CULM 1 encoding a cinnamyl-alcohol dehydrogenase controls culm mechanical strength in rice.

    abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9448-8

    authors: Li X,Yang Y,Yao J,Chen G,Li X,Zhang Q,Wu C

    更新日期:2009-04-01 00:00:00

  • The plant natriuretic peptide receptor is a guanylyl cyclase and enables cGMP-dependent signaling.

    abstract::The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0465-8

    authors: Turek I,Gehring C

    更新日期:2016-06-01 00:00:00

  • Regulatory cis-elements are located in accessible promoter regions of the CAT2 promoter and affect activating histone modifications in Arabidopsis thaliana.

    abstract::Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0546-8

    authors: Laxa M

    更新日期:2017-01-01 00:00:00

  • Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.

    abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016019

    authors: Pear JR,Ridge N,Rasmussen R,Rose RE,Houck CM

    更新日期:1989-12-01 00:00:00

  • DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli.

    abstract::The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005867215258

    authors: Wu H,Liu XQ

    更新日期:1997-05-01 00:00:00

  • A Brassica napus PHT1 phosphate transporter, BnPht1;4, promotes phosphate uptake and affects roots architecture of transgenic Arabidopsis.

    abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0249-y

    authors: Ren F,Zhao CZ,Liu CS,Huang KL,Guo QQ,Chang LL,Xiong H,Li XB

    更新日期:2014-12-01 00:00:00

  • Cloning and molecular analysis of structural genes involved in anthocyanin biosynthesis and expressed in a forma-specific manner in Perilla frutescens.

    abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005959203396

    authors: Gong Z,Yamazaki M,Sugiyama M,Tanaka Y,Saito K

    更新日期:1997-12-01 00:00:00

  • A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.

    abstract::Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9926-x

    authors: Luo L,Herrin DL

    更新日期:2012-07-01 00:00:00

  • A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity.

    abstract::The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040578

    authors: Kim Y,Buckley K,Costa MA,An G

    更新日期:1994-01-01 00:00:00

  • Cyclophilins are encoded by a small gene family in rice.

    abstract::cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028878

    authors: Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

    更新日期:1994-08-01 00:00:00

  • TATA sequence requirements for the initiation of transcription for an RNA polymerase II in vitro transcription system from Nicotiana tabacum.

    abstract::This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006056128129

    authors: Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

    更新日期:1998-12-01 00:00:00

  • Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants.

    abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1019864222515

    authors: Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

    更新日期:2002-10-01 00:00:00

  • Evaluation of the mature grain phytase candidate HvPAPhy_a gene in barley (Hordeum vulgare L.) using CRISPR/Cas9 and TALENs.

    abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0640-6

    authors: Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

    更新日期:2017-09-01 00:00:00

  • Analysis of type 1 metallothionein cDNAs in Vicia faba.

    abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005790927581

    authors: Foley RC,Liang ZM,Singh KB

    更新日期:1997-03-01 00:00:00

  • Molecular details of tomato extensin and glycine-rich protein gene expression.

    abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027342

    authors: Showalter AM,Butt AD,Kim S

    更新日期:1992-05-01 00:00:00