Abstract:
:The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully developed at 21 days after flowering (DAF), but the levels of nutrients, including carbohydrates, fatty acids, and amino acids, increased rapidly from 21 to 35 DAF. Orthogonal partial least squares discriminant analysis and correlation analysis identified 37 metabolites that correlated closely with seed fresh weight. To determine the influence of silique wall photosynthesis on the metabolites in the funiculus, we also covered the siliques of intact plants with aluminum foil; in these plants, the funiculus and silique wall had lower metabolite levels, compared with control. RNA-sequencing analysis of the funiculi in the dark-treated and light-exposed siliques showed that the expression of genes encoding nutrient transporters significantly increased in the funiculi in the dark-treated siliques. Furthermore, the transcripts encoding primary metabolic enzymes for amino acid synthesis, fatty acid synthesis and triacylglycerol assembly, and sucrose-starch metabolism, were also markedly up-regulated, despite the decline in metabolite levels of funiculi in the dark-treated silique. These results provide new insights into funiculus function in seed growth and synthesis of storage reserves in seeds, at the metabolic and transcriptional levels. The identification of these metabolites and genes also provides useful information for creating genetically enhanced oilseed crops with improved seed properties.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Tan H,Xiang X,Tang J,Wang Xdoi
10.1007/s11103-016-0530-3subject
Has Abstractpub_date
2016-11-01 00:00:00pages
539-553issue
4-5eissn
0167-4412issn
1573-5028pii
10.1007/s11103-016-0530-3journal_volume
92pub_type
杂志文章abstract::The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked b...
journal_title:Plant molecular biology
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doi:10.1007/BF00032673
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abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040617
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00026782
更新日期:1992-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00016474
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9177-4
更新日期:2007-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9204-5
更新日期:2007-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006419232075
更新日期:2000-05-01 00:00:00
abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019864222515
更新日期:2002-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0684-7
更新日期:2018-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037068
更新日期:1991-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Plant molecular biology
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doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1997-07-01 00:00:00
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更新日期:2003-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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journal_title:Plant molecular biology
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更新日期:2018-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2010-04-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2007-01-01 00:00:00
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更新日期:2020-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020398
更新日期:1995-06-01 00:00:00