Distinct reorganization of the genome transcription associates with organogenesis of somatic embryo, shoots, and roots in rice.

Abstract:

:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture system to generate somatic embryos, which were further induced into shoots and roots. The global transcriptional reorganization during the development of somatic embryos, shoots, and roots from cultured cells was studied using a rice whole genome microarray and verified by RNA blotting analysis of representative genes. Overall, only 1-3% of expressed genes were differentially regulated during each organogenesis process at the examined time point. Also metabolic pathways were minimally regulated. Thus the genes that dictating organ formation should be relatively small in number. Comparison of these three transcriptomes revealed little overlap during these three organogenesis processes. These results indicate that each organogenesis involves specific reorganization of genome expression.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

doi

10.1007/s11103-006-9092-0

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

337-49

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

63

pub_type

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