Abstract:
:A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the observed length heterogeneity is due to homologous recombination.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Luschnig C,Bachmair A,Schweizer Ddoi
10.1007/BF00015983subject
Has Abstractpub_date
1993-06-01 00:00:00pages
543-5issue
3eissn
0167-4412issn
1573-5028journal_volume
22pub_type
杂志文章abstract::Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9819-4
更新日期:2011-11-01 00:00:00
abstract::The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9344-2
更新日期:2008-08-01 00:00:00
abstract::The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005959203396
更新日期:1997-12-01 00:00:00
abstract::Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-1004-1
更新日期:2005-11-01 00:00:00
abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-008-9374-9
更新日期:2009-03-01 00:00:00
abstract::Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00911-0
更新日期:2019-11-01 00:00:00
abstract::We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005833216479
更新日期:1997-10-01 00:00:00
abstract::Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1013644026132
更新日期:2001-12-01 00:00:00
abstract:KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0799-5
更新日期:2019-01-01 00:00:00
abstract::DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039524
更新日期:1994-10-01 00:00:00
abstract::Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9399-0
更新日期:2008-12-01 00:00:00
abstract::Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019553
更新日期:1996-03-01 00:00:00
abstract::The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017921
更新日期:1991-01-01 00:00:00
abstract::Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006176819213
更新日期:1999-03-01 00:00:00
abstract::We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005818507743
更新日期:1997-11-01 00:00:00
abstract:KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00870-6
更新日期:2019-07-01 00:00:00
abstract::The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037145
更新日期:1991-11-01 00:00:00
abstract::A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005704616565
更新日期:1997-03-01 00:00:00
abstract::We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019948
更新日期:1993-01-01 00:00:00
abstract:KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01018-7
更新日期:2020-08-01 00:00:00
abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5101-y
更新日期:2005-06-01 00:00:00
abstract::Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041174
更新日期:1995-11-01 00:00:00
abstract::A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00034937
更新日期:1986-09-01 00:00:00
abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040674
更新日期:1992-02-01 00:00:00
abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1012457113700
更新日期:2001-11-01 00:00:00
abstract::Electrophoretic analysis of translation products of polyadenylated RNA isolated from mid-maturation sorghum seed in the presence of [(35)S]met, [(3)H]leu, or [(3)H]val revealed two major proteins of kDa and 21 kDa. These products were not detected when [(3)H]lys was supplied as the radioactive substrate. Under similar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043202
更新日期:1989-03-01 00:00:00
abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1005816823636
更新日期:1997-12-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0069-5
更新日期:2013-07-01 00:00:00