Abstract：
:RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in green and non-green seedlings (etiolated, cia5-2, ispF and ispG albino mutants, lincomycin-, and norflurazon-treated). The editing efficiency of Arabidopsis plastid transcripts varies from site to site, and may be specifically regulated in different tissues. Steady state levels of plastid transcripts are low or undetectable in etiolated seedlings, but most editing sites are edited with efficiencies similar to those observed in green seedlings. By contrast, the editing of some sites is completely lost or significantly reduced in other non-green tissues; for instance, the editing of ndhB-149, ndhB-1255, and ndhD-2 is completely lost in roots and in lincomycin-treated seedlings. The editing of ndhD-2 is also completely lost in albino mutants and norflurazon-treated seedlings. However, matK-640 is completely edited, and accD-794, atpF-92, psbE-214, psbF-77, psbZ-50, and rps14-50 are completely or highly edited in both green and non-green tissues. In addition, the expression of nucleus-encoded RNA polymerase dependent transcripts is specifically induced by lincomycin, and the splicing of ndhB transcripts is significantly reduced in the albino mutants and inhibitor-treated seedlings. Our results indicate that plastid gene expression, and the splicing and editing of plastid transcripts are specifically and differentially regulated in various types of non-green tissues.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Tseng CC,Lee CJ,Chung YT,Sung TY,Hsieh MHdoi
10.1007/s11103-013-0069-5subject
Has Abstractpub_date
2013-07-01 00:00:00pages
375-92issue
4-5eissn
0167-4412issn
1573-5028journal_volume
82pub_type
杂志文章abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040527
更新日期：1992-09-01 00:00:00
abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026494813936
更新日期：1999-05-01 00:00:00
abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023618
更新日期：1993-03-01 00:00:00
abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006178710443
更新日期：1999-01-01 00:00:00
abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005909703600
更新日期：1998-05-01 00:00:00
abstract：:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9240-1
更新日期：2007-12-01 00:00:00
abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0240-7
更新日期：2014-11-01 00:00:00
abstract：KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0595-7
更新日期：2017-05-01 00:00:00
abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027351
更新日期：1992-05-01 00:00:00
abstract：:Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0087-3
更新日期：2013-11-01 00:00:00
abstract：:Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0526-z
更新日期：2016-11-01 00:00:00
abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0239-0
更新日期：2014-11-01 00:00:00
abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006499832424
更新日期：2000-06-01 00:00:00
abstract：:The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006464822434
更新日期：2000-09-01 00:00:00
abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015653
更新日期：1990-01-01 00:00:00
abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041395
更新日期：1996-12-01 00:00:00
abstract：:Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006204318499
更新日期：1999-07-01 00:00:00
abstract：:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028864
更新日期：1994-11-01 00:00:00
abstract：:Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015080
更新日期：1991-05-01 00:00:00
abstract：:Several clones corresponding to the gene encoding NADH-dependent hydroxypyruvate reductase have been isolated from a cucumber genomic library. Restriction mapping indicates the presence of two HPR genes, hpr-A and hpr-B, in the cucumber genome. Examination of the DNAs of individual plants suggests that hpr-A and hpr-B...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037078
更新日期：1991-10-01 00:00:00
abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0244-3
更新日期：2014-11-01 00:00:00
abstract：:Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006338401808
更新日期：1999-11-01 00:00:00
abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019126
更新日期：1995-10-01 00:00:00
abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:Repetitive DNA sequences comprise a large percentage of plant genomes, and their characterization provides information about both species and genome evolution. We have isolated a recombinant clone containing a highly repeated DNA element (SB92) that is homologous to ca. 0.9% of the soybean genome or about 10(5) copies...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041174
更新日期：1995-11-01 00:00:00
abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027384
更新日期：1988-05-01 00:00:00
abstract：:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the exp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019157
更新日期：1990-09-01 00:00:00
abstract：:Pathogens induce the expression of many genes encoding plant transcription factors, though specific knowledge of the biological function of individual transcription factors remains scarce. NAC transcription factors are encoded in plants by a gene family with proposed functions in both abiotic and biotic stress adaptat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9204-5
更新日期：2007-09-01 00:00:00
abstract：:The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016430
更新日期：1987-01-01 00:00:00
abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-0054-3
更新日期：2006-08-01 00:00:00
