Abstract:
:Mitogen-activated protein kinases (MAPKs) constitute one of the most critical signaling components in plants. A typical example is wound-induced protein kinase (WIPK), which functions during pathogen responses in tobacco plants (Nicotiana tabacum). Searching for direct down-stream components, we previously isolated a novel transcription factor, which was activated upon phosphorylation by WIPK and designated as N. tabacum WIPK-interacting factor (NtWIF). Overexpression of NtWIF in tobacco plants enhanced the hypersensitive response (HR) upon tobacco mosaic virus infection and cryptogein treatment, while its silencing by RNAi suppressed such HR. NtWIF contains a specific motif similar to the B3 DNA binding domain, which recognizes the core TGTCTC motif called the auxin-responsive element (ARE). Using synthetic ARE sequences, NtWIF was also shown to recognize the ARE motifs and to transactivate the Luciferase (Luc)-reporter gene driven by such AREs in tobacco BY2 cultured cells. Subsequent microarray screening of NtWIF overexpressing tobacco identified 49 stress-responsive genes, and in silico analyses of available promoter regions of these genes revealed beta-1,3-glucanase, ACS2, P-450, and WIPK itself to contain the ARE core motif consisted of either TGTCTC or TGTCCT. Gel shift assay showed NtWIF to efficiently bind to both sequences. Assays with 1.5-kb PR-Q and 1.2 kb WIPK promoter regions, each fused to the Luc-reporter gene, indicated NtWIF to exhibit a clear transactivation activity, which was increased up to 3-fold upon phosphorylation by WIPK. These results revealed that NtWIF directly regulates multiple stress-responsive genes containing the ARE motif in their promoters, thereby partly filling up the last step of the MAPK cascade.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Chung KM,Sano Hdoi
10.1007/s11103-007-9240-1subject
Has Abstractpub_date
2007-12-01 00:00:00pages
763-73issue
6eissn
0167-4412issn
1573-5028journal_volume
65pub_type
杂志文章abstract::Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040527
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journal_title:Plant molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1999-07-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023557
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-07-01 00:00:00
abstract::Leafy spurge (Euphorbia esula) is an herbaceous perennial weed that produces vegetatively from an abundance of underground adventitious buds. In this study, we report the effects of different environmental conditions on vegetative production and flowering competence, and determine molecular mechanisms associated with ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9621-8
更新日期:2010-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
abstract::Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-...
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pub_type: 杂志文章
doi:10.1023/a:1005904230755
更新日期:1998-03-01 00:00:00
abstract::As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006081009173
更新日期:1998-11-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
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更新日期:2001-12-01 00:00:00
abstract::A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...
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更新日期:1999-11-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:1992-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020199
更新日期:1996-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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更新日期:2009-03-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011848528377
更新日期:2001-11-01 00:00:00