Abstract:
:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analysis of one of them, named BnM3.4, by in situ hybridization, showed that it is expressed specifically and at a high level in the rapeseed microspore. The specificity in its profile of expression is most likely transcriptionally controlled as a similar pattern of expression was also observed in Arabidopsis thaliana plants transformed by the BnM3.4 promoter fused to the reporter GUS-coding sequence. The putative BnM3.4 promoter contains three dispersed copies of a motif described previously in the promoters of several genes expressed in the male gametophyte. The BnM3.4 gene encodes a predicted novel proline-rich protein of 23.4 kDa which may interact with cytoskeletal components or have a structural role in the cell wall.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Fourgoux-Nicol A,Drouaud J,Haouazine N,Pelletier G,Guerche Pdoi
10.1023/a:1006282507095subject
Has Abstractpub_date
1999-07-01 00:00:00pages
857-72issue
5eissn
0167-4412issn
1573-5028journal_volume
40pub_type
杂志文章abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2000-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016064
更新日期:1984-05-01 00:00:00
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更新日期:2007-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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更新日期:1990-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020112
更新日期:1996-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027384
更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 已发布勘误
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更新日期:2020-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1005915507497
更新日期:1998-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9445-y
更新日期:2009-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023430
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022090916446
更新日期:2003-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01578377
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023238
更新日期:1994-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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