Abstract:
:A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ZmCIPK16 interacted with ZmCBL3, ZmCBL4, ZmCBL5, and ZmCBL8. Bimolecular fluorescence complementation (BiFC) assays prove that ZmCIPK16 can interact with ZmCBL3, ZmCBL4, ZmCBL5, and ZmCBL8 in vivo. Subcellular localization showed that ZmCIPK16 is distributed in the nucleus, plasma membrane and cytoplasm; this is different from the specific localization of ZmCBL3, ZmCBL4, and ZmCBL5, which are found in the plasma membrane. The results also showed that overexpression of ZmCIPK16 in the Arabidopsis sos2 mutant induced the expression of the SOS1 gene and enhanced salt tolerance. These findings indicate that ZmCIPK16 may be involved in the CBL-CIPK signaling network in maize responses to salt stress.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zhao J,Sun Z,Zheng J,Guo X,Dong Z,Huai J,Gou M,He J,Jin Y,Wang J,Wang Gdoi
10.1007/s11103-008-9445-ysubject
Has Abstractpub_date
2009-04-01 00:00:00pages
661-74issue
6eissn
0167-4412issn
1573-5028journal_volume
69pub_type
杂志文章abstract::We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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