Abstract：
:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we analyzed the mode of interaction between the N factor, a tobacco R factor, and the helicase domain (p50) of tobacco mosaic virus (TMV). To this end, domain dissected proteins were prepared and subjected to Agroinfiltration into intact leaves, followed by yeast two hybrid and pull-down assays. The results pointed to three novel features. First, the N factor was found to directly bind to the p50 of TMV, second, ATP was pre-requisite for this interaction, with formation of an ATP/N factor complex, and third, the N factor was shown to possess ATPase activity, which is enhanced by the p50. Moreover, we found that intra- and/or inter-molecular interactions take place in the N factor molecule. This interaction required ATP, and was disrupted by the p50. Based on these results, we propose a following model for the TMV recognition mechanism in tobacco plants. The N factor forms a complex with ATP, to which the helicase domain interacts, and enhances ATP hydrolysis. The resulting ADP/N factor complex then changes its conformation, thereby facilitating further interaction with the down-stream signaling factor(s). This model is consistent with the idea of 'protein machine'.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ueda H,Yamaguchi Y,Sano Hdoi
10.1007/s11103-005-5817-8subject
Has Abstractpub_date
2006-05-01 00:00:00pages
31-45issue
1-2eissn
0167-4412issn
1573-5028journal_volume
61pub_type
杂志文章abstract：:Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0249-y
更新日期：2014-12-01 00:00:00
abstract：:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028773
更新日期：1990-03-01 00:00:00
abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005867215258
更新日期：1997-05-01 00:00:00
abstract：:A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005849202731
更新日期：1997-07-01 00:00:00
abstract：:The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9138-y
更新日期：2007-05-01 00:00:00
abstract：:Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9722-4
更新日期：2011-02-01 00:00:00
abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006040306581
更新日期：1998-10-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0240-7
更新日期：2014-11-01 00:00:00
abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：1997-09-01 00:00:00
abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006030007333
更新日期：1998-08-01 00:00:00
abstract：:We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027072
更新日期：1992-08-01 00:00:00
abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-6268-6
更新日期：2006-05-01 00:00:00
abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9458-1
更新日期：2009-05-01 00:00:00
abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1011848528377
更新日期：2001-11-01 00:00:00
abstract：:Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0187-8
更新日期：2014-06-01 00:00:00
abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041405
更新日期：1996-12-01 00:00:00
abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006296422485
更新日期：1999-08-01 00:00:00
abstract：:The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006493021557
更新日期：2001-01-01 00:00:00
abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014831302392
更新日期：2002-03-01 00:00:00
abstract：:Drought is one of the critical factors limiting reproductive yields of rice and other crops globally. However, little is known about the molecular mechanism underlying reproductive development under drought stress in rice. To explore the potential gene function for improving rice reproductive development under drought...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0057-9
更新日期：2013-06-01 00:00:00
abstract：:Tandemly arrayed genes (TAGs) account for about one-third of the duplicated genes in eukaryotic genomes. They provide raw genetic material for biological evolution, and play important roles in genome evolution. The 22-kDa prolamin genes in cereal genomes represent typical TAG organization, and provide the good materia...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9705-5
更新日期：2010-12-01 00:00:00
abstract：:The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019303
更新日期：1993-11-01 00:00:00
abstract：:A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020464
更新日期：1995-12-01 00:00:00
abstract：:Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006498413543
更新日期：2000-09-01 00:00:00
abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9780-2
更新日期：2011-05-01 00:00:00
abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0601-0
更新日期：2017-05-01 00:00:00
abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020486
更新日期：1996-12-01 00:00:00
abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006232700835
更新日期：1999-09-01 00:00:00
abstract：:Evidence is emerging that the E2F family of transcription factors plays an important role in the regulation of gene expression at the G1/S transition in plants. Here, we show that in the tobacco proliferating cell nuclear antigen (PCNA), whose transcript is specifically expressed at G1/S phase, the two E2F binding sit...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-6601-x
更新日期：2005-01-01 00:00:00
