Functional analysis of a RPD3 histone deacetylase homologue in Arabidopsis thaliana.

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00

abstract：:The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

journal_title：Plant molecular biology

authors： Grimmig B,Matern U

更新日期：1997-01-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

journal_title：Plant molecular biology

authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

更新日期：2009-02-01 00:00:00

abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...

journal_title：Plant molecular biology

authors： Farré JC,Araya A

更新日期：1999-08-01 00:00:00

abstract：:This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...

journal_title：Plant molecular biology

authors： Yamaguchi Y,Itoh Y,Takeda Y,Yamazaki K

更新日期：1998-12-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

journal_title：Plant molecular biology

authors： Morel JB,Vaucheret H

更新日期：2000-06-01 00:00:00

abstract：:Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...

journal_title：Plant molecular biology

authors： Chapman KA,Delauney AJ,Kim JH,Verma DP

更新日期：1994-01-01 00:00:00

abstract：:β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

journal_title：Plant molecular biology

authors： Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

更新日期：2012-02-01 00:00:00

abstract：:The synthesis of both strands of CaMV-DNA has been studied in vitro using viral replication complexes obtained by hypotonic extraction of infected plant organelles. Hybridization of the DNA synthesized in vitro to single stranded CaMV DNA probes cloned in bacteriophage M 13 confirmed that the 35 S RNA served as a temp...

journal_title：Plant molecular biology

authors： Pfeiffer P,Laquel P,Hohn T

更新日期：1984-09-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

journal_title：Plant molecular biology

authors： Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

更新日期：2016-01-01 00:00:00

abstract：KEY MESSAGE:Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls. Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor,...

journal_title：Plant molecular biology

authors： Matsuoka K,Yanagi R,Yumoto E,Yokota T,Yamane H,Satoh S,Asahina M

更新日期：2018-04-01 00:00:00

abstract：:Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...

journal_title：Plant molecular biology

authors： Close TJ,Fenton RD,Moonan F

更新日期：1993-10-01 00:00:00

abstract：:Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...

journal_title：Plant molecular biology

authors： Schlagnhaufer CD,Arteca RN,Pell EJ

更新日期：1997-12-01 00:00:00

abstract：:A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...

journal_title：Plant molecular biology

authors： Kojima K,Yamamoto N,Sasaki S

更新日期：1992-06-01 00:00:00

abstract：:In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell...

journal_title：Plant molecular biology

authors： Tardieu F,Granier C

更新日期：2000-08-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00

abstract：:The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...

journal_title：Plant molecular biology

authors： Oung HM,Lin KC,Wu TM,Chandrika NN,Hong CY

更新日期：2015-12-01 00:00:00

abstract：KEY MESSAGE:Arabidopsis chloroplast RNase J displaces both exo- and endo-ribonucleolytic activities and contains a unique GT-1 DNA binding domain. Control of chloroplast gene expression is predominantly at the post-transcriptional level via the coordinated action of nuclear encoded ribonucleases and RNA-binding protein...

journal_title：Plant molecular biology

authors： Halpert M,Liveanu V,Glaser F,Schuster G

更新日期：2019-01-01 00:00:00

abstract：:In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...

journal_title：Plant molecular biology

authors： Piechota J,Bereza M,Sokołowska A,Suszyński K,Lech K,Jańska H

更新日期：2015-06-01 00:00:00

abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

journal_title：Plant molecular biology

authors： Sakamoto K,Nagatani A

更新日期：1996-08-01 00:00:00

abstract：:We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and i...

journal_title：Plant molecular biology

authors： Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein H

更新日期：1994-10-01 00:00:00

abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...

journal_title：Plant molecular biology

authors： Tang W,Sun J,Liu J,Liu F,Yan J,Gou X,Lu BR,Liu Y

更新日期：2014-11-01 00:00:00

abstract：:Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...

journal_title：Plant molecular biology

authors： Manninen I,Schulman AH

更新日期：1993-08-01 00:00:00

abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

journal_title：Plant molecular biology

authors： Panpetch P,Field RA,Limpaseni T

更新日期：2018-03-01 00:00:00

abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

journal_title：Plant molecular biology

authors： Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

更新日期：2011-05-01 00:00:00

abstract：:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...

journal_title：Plant molecular biology

authors： Kim MJ,Ham BK,Kim HR,Lee IJ,Kim YJ,Ryu KH,Park YI,Paek KH

更新日期：2005-12-01 00:00:00

abstract：:Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...

journal_title：Plant molecular biology

authors： Xie Q,Sanz-Burgos AP,Guo H,García JA,Gutiérrez C

更新日期：1999-03-01 00:00:00