In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and cucumber mosaic virus proteins.

Abstract:

:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Kim MJ,Ham BK,Kim HR,Lee IJ,Kim YJ,Ryu KH,Park YI,Paek KH

doi

10.1007/s11103-005-2619-y

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

981-94

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

59

pub_type

杂志文章
  • Isolation of an Arabidopsis homologue of the maize homeobox Knotted-1 gene.

    abstract::The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021797

    authors: Granger CL,Callos JD,Medford JI

    更新日期:1996-05-01 00:00:00

  • Structure and expression of the barley lipid transfer protein gene Ltp1.

    abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040674

    authors: Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

    更新日期:1992-02-01 00:00:00

  • CRISPR/Cas9-mediated targeted T-DNA integration in rice.

    abstract:KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-018-00819-1

    authors: Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

    更新日期:2019-03-01 00:00:00

  • Comparison of the expression of two highly homologous members of the soybean ribulose-1,5-bisphosphate carboxylase small subunit gene family.

    abstract::Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019389

    authors: Shirley BW,Ham DP,Senecoff JF,Berry-Lowe SL,Zurfluh LL,Shah DM,Meagher RB

    更新日期:1990-06-01 00:00:00

  • Differential expression profiles of growth-related genes in the elongation zone of maize primary roots.

    abstract::Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3474-y

    authors: Bassani M,Neumann PM,Gepstein S

    更新日期:2004-10-01 00:00:00

  • Identification of cis-acting elements involved in the regulation of the pathogenesis-related gene STH-2 in potato.

    abstract::We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014935

    authors: Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

    更新日期:1993-05-01 00:00:00

  • The expression of chloroplast genes during cotton embryogenesis.

    abstract::The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040137

    authors: Borroto KE,Dure L 3rd

    更新日期:1986-03-01 00:00:00

  • A novel stress-inducible metallothionein-like gene from rice.

    abstract::A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020388

    authors: Hsieh HM,Liu WK,Huang PC

    更新日期:1995-06-01 00:00:00

  • Methyl-coenzyme M reductase-dependent endogenous methane enhances plant tolerance against abiotic stress and alters ABA sensitivity in Arabidopsis thaliana.

    abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00914-x

    authors: Su J,Yang X,He J,Zhang Y,Duan X,Wang R,Shen W

    更新日期:2019-11-01 00:00:00

  • Characterization of three distinct cDNA clones encoding cysteine proteinases from maize (Zea mays L.) callus.

    abstract::In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1026494813936

    authors: Pechan T,Jiang B,Steckler D,Ye L,Lin L,Luthe DS,Williams WP

    更新日期:1999-05-01 00:00:00

  • Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants.

    abstract::We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027495

    authors: Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

    更新日期:1990-04-01 00:00:00

  • Information contents and dinucleotide compositions of plant intron sequences vary with evolutionary origin.

    abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/BF00040537

    authors: White O,Soderlund C,Shanmugan P,Fields C

    更新日期:1992-09-01 00:00:00

  • Transcription and translation of heat shock and normal proteins in seedlings and developing seeds of soybean exposed to a gradual temperature increase.

    abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020626

    authors: Altschuler M,Mascarenhas JP

    更新日期:1985-09-01 00:00:00

  • Lignin synthesized by CmCAD2 and CmCAD3 in oriental melon (Cucumis melo L.) seedlings contributes to drought tolerance.

    abstract:KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01018-7

    authors: Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

    更新日期:2020-08-01 00:00:00

  • OsPRP3, a flower specific proline-rich protein of rice, determines extracellular matrix structure of floral organs and its overexpression confers cold-tolerance.

    abstract::Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9557-z

    authors: Gothandam KM,Nalini E,Karthikeyan S,Shin JS

    更新日期:2010-01-01 00:00:00

  • Evidence implying only unprimed RdRP activity during transitive gene silencing in plants.

    abstract::RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-7307-4

    authors: Petersen BO,Albrechtsen M

    更新日期:2005-07-01 00:00:00

  • Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea.

    abstract:KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00890-2

    authors: Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

    更新日期:2019-09-01 00:00:00

  • Chemical synthesis, expression and mutagenesis of a gene encoding beta-cryptogein, an elicitin produced by Phytophthora cryptogea.

    abstract::Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019323

    authors: O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JC

    更新日期:1995-02-01 00:00:00

  • A novel E-type endo-beta-1,4-glucanase with a putative cellulose-binding domain is highly expressed in ripening strawberry fruits.

    abstract::Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006299821980

    authors: Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

    更新日期:1999-05-01 00:00:00

  • Characterization of rps17, rp19 and rpl15: three nucleus-encoded plastid ribosomal protein genes.

    abstract::Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019207

    authors: Thompson MD,Jacks CM,Lenvik TR,Gantt JS

    更新日期:1992-03-01 00:00:00

  • Producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA.

    abstract::In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9785-x

    authors: Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

    更新日期:2011-08-01 00:00:00

  • The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice.

    abstract:KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01010-1

    authors: Liu X,Wu D,Shan T,Xu S,Qin R,Li H,Negm M,Wu D,Li J

    更新日期:2020-07-01 00:00:00

  • A pathogen-induced gene of barley encodes a HSP90 homologue showing striking similarity to vertebrate forms resident in the endoplasmic reticulum.

    abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00023606

    authors: Walther-Larsen H,Brandt J,Collinge DB,Thordal-Christensen H

    更新日期:1993-03-01 00:00:00

  • Leaf initiation: the integration of growth and cell division.

    abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-005-7703-9

    authors: Fleming AJ

    更新日期:2006-04-01 00:00:00

  • New features of desiccation tolerance in the lichen photobiont Trebouxia gelatinosa are revealed by a transcriptomic approach.

    abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0468-5

    authors: Carniel FC,Gerdol M,Montagner A,Banchi E,De Moro G,Manfrin C,Muggia L,Pallavicini A,Tretiach M

    更新日期:2016-06-01 00:00:00

  • Constitutive and light-induced DNAseI hypersensitive sites in the rbcS genes of pea (Pisum sativum).

    abstract::The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017456

    authors: Görz A,Schäfer W,Hirasawa E,Kahl G

    更新日期:1988-09-01 00:00:00

  • Nutritional functions of the funiculus in Brassica napus seed maturation revealed by transcriptome and dynamic metabolite profile analyses.

    abstract::The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-016-0530-3

    authors: Tan H,Xiang X,Tang J,Wang X

    更新日期:2016-11-01 00:00:00

  • Molecular details of tomato extensin and glycine-rich protein gene expression.

    abstract::In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027342

    authors: Showalter AM,Butt AD,Kim S

    更新日期:1992-05-01 00:00:00

  • OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.

    abstract::Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9752-6

    authors: Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

    更新日期:2011-04-01 00:00:00

  • Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2.

    abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021807

    authors: Burow MD,Chen ZY,Mouton TM,Moroney JV

    更新日期:1996-05-01 00:00:00