Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants.

Abstract:

:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tissue-specific and positively affected by light. Response times, both to dark and light conditions, are remarkably rapid. A chimeric gene consisting of a 1.2 kb Fd promoter fragment fused to the beta-glucuronidase reporter gene was transferred to tobacco. This fusion gene is expressed in a tissue-specific way; it shows high levels of expression in green leaves, as compared to root tissue.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

doi

10.1007/BF00027495

subject

Has Abstract

pub_date

1990-04-01 00:00:00

pages

491-9

issue

4

eissn

0167-4412

issn

1573-5028

journal_volume

14

pub_type

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