Abstract:
:More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II. One of these CTD-phosphatase-like (CPL) proteins, CPL2, dephosphorylates CTD-Ser5-PO4 in an intact RNA polymerase II complex and contains a double-stranded (ds)-RNA-binding motif (DRM). Although the dsRNA-binding activity of CPL2 DRM has not been shown to date, T-DNA insertion mutants that express CPL2 variants lacking either a part of DRM (cpl2-1) or the entire DRM (cpl2-2) exhibited leaf expansion defects, early flowering, low fertility, and increased salt sensitivity. cpl2 mutant plants produced shorter hypocotyls than wild-type plants in the light, but were indistinguishable from wild type in the dark. CPL2 was expressed in shoot and root meristems and vasculatures, expanding rosette leaves, and floral organs suggesting a focal role for growth. Microarray and RT-PCR analyses revealed that basal levels of several auxin-responsive transcripts were reduced in cpl2. On the other hand, the levels of endogenous auxin and its conjugates were similar in wild type and cpl2. Overexpression of ARF5 but not all activator ARF transcription factors restored the auxin-responsive DR5-GUS reporter gene expression and the leaf expansion of cpl2 mutant plants but not early flowering phenotype. These results establish CPL2 as a multifunctional regulator that modulates plant growth, stress, and auxin responses.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ueda A,Li P,Feng Y,Vikram M,Kim S,Kang CH,Kang JS,Bahk JD,Lee SY,Fukuhara T,Staswick PE,Pepper AE,Koiwa Hdoi
10.1007/s11103-008-9348-ysubject
Has Abstractpub_date
2008-08-01 00:00:00pages
683-97issue
6eissn
0167-4412issn
1573-5028journal_volume
67pub_type
杂志文章abstract::The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019218
更新日期:1992-03-01 00:00:00
abstract:KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0542-z
更新日期:2016-12-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9694-4
更新日期:2010-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00981-5
更新日期:2020-05-01 00:00:00
abstract::The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006058700720
更新日期:1998-06-01 00:00:00
abstract::We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027495
更新日期:1990-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-07-01 00:00:00
abstract::A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015079
更新日期:1991-05-01 00:00:00
abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006196530079
更新日期:1999-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0239-0
更新日期:2014-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019851913083
更新日期:2002-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010645431133
更新日期:2001-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0563-7
更新日期:2017-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00945-4
更新日期:2020-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037051
更新日期:1991-10-01 00:00:00
abstract::Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9635-2
更新日期:2010-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0210-0
更新日期:2014-09-01 00:00:00
abstract::A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027135
更新日期:1986-11-01 00:00:00
abstract::We describe a non-LTR retrotransposon family,BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9542-6
更新日期:2009-12-01 00:00:00
abstract::Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0082-8
更新日期:2013-10-01 00:00:00
abstract::Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006199003756
更新日期:1999-01-01 00:00:00
abstract::A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042240
更新日期:1996-06-01 00:00:00
abstract::Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015670
更新日期:1988-03-01 00:00:00