当前位置： SCI文献检索 > PLANT MOLECULAR BIOLOGY期刊下所有文献 > The tomato brassinosteroid receptor BRI1 increases binding of systemin to tobacco plasma membranes, but is not involved in systemin signaling.

The tomato brassinosteroid receptor BRI1 increases binding of systemin to tobacco plasma membranes, but is not involved in systemin signaling.

Abstract：

:The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant cu3 is as sensitive to systemin as wild type plants. Here we explored these contradictory findings by studying the responses of tobacco plants (Nicotiana tabacum) to systemin. A fluorescently-labeled systemin analog bound specifically to plasma membranes of tobacco suspension-cultured cells that expressed the tomato BRI1-FLAG transgene, but not to wild type tobacco cells. On the other hand, signaling responses to systemin, such as activation of mitogen-activated protein kinases and medium alkalinization, were neither increased in BRI1-FLAG-overexpressing tobacco cells nor decreased in BRI1-silenced cells as compared to levels in untransformed control cells. Furthermore, in transgenic tobacco plants BRI1-FLAG became phosphorylated on threonine residues in response to brassinolide application, but not in response to systemin. When BRI1 transcript levels were reduced by virus-induced gene silencing in tomato plants, the silenced plants displayed a phenotype characteristic of bri1 mutants. However, their response to overexpression of the Prosystemin transgene was the same as in control plants. Taken together, our data suggest that BRI1 can function as a systemin binding protein, but that binding of the ligand does not transduce the signal into the cell. This unusual behavior and the nature of the elusive systemin receptor will be discussed.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Malinowski R,Higgins R,Luo Y,Piper L,Nazir A,Bajwa VS,Clouse SD,Thompson PR,Stratmann JW

doi

10.1007/s11103-009-9494-x

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

603-16

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

70

pub_type

杂志文章

相关文献

PLANT MOLECULAR BIOLOGY文献大全
  • Cloning of two plant cDNAs encoding a beta-type proteasome subunit and a transformer-2-like SR-related protein: early induction of the corresponding genes in tobacco cells treated with cryptogein.

    abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005833216479

    authors： Petitot AS,Blein JP,Pugin A,Suty L

    更新日期：1997-10-01 00:00:00

  • Down-regulation of specific members of the glutamine synthetase gene family in alfalfa by antisense RNA technology.

    abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006099512706

    authors： Temple SJ,Bagga S,Sengupta-Gopalan C

    更新日期：1998-06-01 00:00:00

  • Phylogenetic origin of a secondary pathway: the case of pyrrolizidine alkaloids.

    abstract：:Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...

    journal_title：Plant molecular biology

    pub_type： 杂志文章,评审

    doi：10.1023/a:1026597621646

    authors： Ober D,Hartmann T

    更新日期：2000-11-01 00:00:00

  • Primer extension studies on alpha-amylase mRNAs in barley aleurone. I. Characterization and quantification of the transcripts.

    abstract：:Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00023427

    authors： Chandler PM,Huiet L

    更新日期：1991-04-01 00:00:00

  • Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.

    abstract：:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-006-9078-y

    authors： Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev A

    更新日期：2007-01-01 00:00:00

  • Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme.

    abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00019506

    authors： Henze K,Schnarrenberger C,Kellermann J,Martin W

    更新日期：1994-12-01 00:00:00

  • Correction to: Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.

    abstract：:Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

    journal_title：Plant molecular biology

    pub_type： 已发布勘误

    doi：10.1007/s11103-020-00970-8

    authors： Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

    更新日期：2020-03-01 00:00:00

  • Tobacco-expressed Brassica juncea chitinase BjCHI1 shows antifungal activity in vitro.

    abstract：:We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1016067200148

    authors： Fung KL,Zhao KJ,He ZM,Chye ML

    更新日期：2002-09-01 00:00:00

  • A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP and SSR markers.

    abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1014831302392

    authors： Menz MA,Klein RR,Mullet JE,Obert JA,Unruh NC,Klein PE

    更新日期：2002-03-01 00:00:00

  • Transcription and translation of heat shock and normal proteins in seedlings and developing seeds of soybean exposed to a gradual temperature increase.

    abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00020626

    authors： Altschuler M,Mascarenhas JP

    更新日期：1985-09-01 00:00:00

  • A GARP transcription factor anther dehiscence defected 1 (OsADD1) regulates rice anther dehiscence.

    abstract：:Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice m...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-019-00911-0

    authors： Xiao Y,You S,Kong W,Tang Q,Bai W,Cai Y,Zheng H,Wang C,Jiang L,Wang C,Zhao Z,Wan J

    更新日期：2019-11-01 00:00:00

  • Stress response of transgenic tobacco plants expressing a cyanobacterial ferredoxin in chloroplasts.

    abstract：:Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-011-9786-9

    authors： Ceccoli RD,Blanco NE,Medina M,Carrillo N

    更新日期：2011-08-01 00:00:00

  • ASYMMETRIC LEAVES2-LIKE1 gene, a member of the AS2/LOB family, controls proximal-distal patterning in Arabidopsis petals.

    abstract：:The formation and the development of the floral organs require an intercalate expression of organ-specific genes. At the same time, meristem-specific genes are repressed to complete the differentiation of the organs in the floral whorls. In an Arabidopsis activation tagging population, a mutant affected in inflorescen...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-005-0698-4

    authors： Chalfun-Junior A,Franken J,Mes JJ,Marsch-Martinez N,Pereira A,Angenent GC

    更新日期：2005-03-01 00:00:00

  • Characterization of a Euglena gene encoding a polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II.

    abstract：:A 7.4 kb segment of a Euglena nuclear gene encoding a portion of the polyprotein precursor to the light-harvesting chlorophyll a/b-binding protein of photosystem II (LHCPII) has been isolated and sequenced. Nine exons can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of a...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00034956

    authors： Muchhal US,Schwartzbach SD

    更新日期：1992-01-01 00:00:00

  • Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis.

    abstract：:The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006341210147

    authors： Bischoff F,Vahlkamp L,Molendijk A,Palme K

    更新日期：2000-02-01 00:00:00

  • A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine.

    abstract：:A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/B:PLAN.0000009269.97773.70

    authors： Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama K

    更新日期：2003-09-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-008-9296-6

    authors： Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期：2008-04-01 00:00:00

  • CaMADS1, a MADS box gene expressed in the carpel of hazelnut.

    abstract：:Hazelnut (Corylus avellana L.) is a species of economic interest that shows a peculiar floral biology. Unlike most of the angiosperms, which produce ovules during floral development such that they are ready for pollen at anthesis, hazelnut ovary development is delayed and triggered by compatible pollination. In order ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1006022524708

    authors： Rigola D,Pè ME,Fabrizio C,Mè G,Sari-Gorla M

    更新日期：1998-12-01 00:00:00

  • OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.

    abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-011-9752-6

    authors： Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

    更新日期：2011-04-01 00:00:00

  • Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

    abstract：:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00028867

    authors： Conceição Ada S,Van Vliet A,Krebbers E

    更新日期：1994-11-01 00:00:00

  • DNA binding and bending by a chloroplast-encoded HU-like protein overexpressed in Escherichia coli.

    abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005867215258

    authors： Wu H,Liu XQ

    更新日期：1997-05-01 00:00:00

  • Functional analysis of a complex oncogene arrangement in biotype III Agrobacterium tumefaciens strains.

    abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00018558

    authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

    更新日期：1990-02-01 00:00:00

  • Genome-wide transcriptomic analysis of response to low temperature reveals candidate genes determining divergent cold-sensitivity of maize inbred lines.

    abstract：:Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-014-0187-8

    authors： Sobkowiak A,Jończyk M,Jarochowska E,Biecek P,Trzcinska-Danielewicz J,Leipner J,Fronk J,Sowiński P

    更新日期：2014-06-01 00:00:00

  • Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana.

    abstract：:Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1016047231597

    authors： Osakabe K,Yoshioka T,Ichikawa H,Toki S

    更新日期：2002-09-01 00:00:00

  • Differential screening of mitochondrial cDNA libraries from male-fertile and cytoplasmic male-sterile sugar-beet reveals genome rearrangements at atp6 and atpA loci.

    abstract：:As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00024201

    authors： Xue Y,Collin S,Davies DR,Thomas CM

    更新日期：1994-04-01 00:00:00

  • Transcriptional dynamics during cell wall removal and regeneration reveals key genes involved in cell wall development in rice.

    abstract：:Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/s11103-011-9819-4

    authors： Sharma R,Tan F,Jung KH,Sharma MK,Peng Z,Ronald PC

    更新日期：2011-11-01 00:00:00

  • Flow cytometric analysis of tobacco and cowpea protoplasts infected in vivo and in vitro with alfalfa mosaic virus.

    abstract：:Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00187572

    authors： van Klaveren P,Slats J,Roosien J,van Vloten-Doting L

    更新日期：1983-01-01 00:00:00

  • Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.

    abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00041395

    authors： Kim J,Leustek T

    更新日期：1996-12-01 00:00:00

  • Characterisation and promoter analysis of the Arabidopsis gene encoding high-mobility-group protein HMG-I/Y.

    abstract：:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1023/a:1005928219895

    authors： Gupta R,Webster CI,Gray JC

    更新日期：1998-04-01 00:00:00

  • Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco.

    abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

    journal_title：Plant molecular biology

    pub_type： 杂志文章

    doi：10.1007/BF00042040

    authors： Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

    更新日期：1995-04-01 00:00:00