Abnormal 'wrinkled' cell walls and retarded development of transgenic Arabidopsis thaliana plants expressing endo-1,4-beta-glucanase (cell) antisense.

abstract：:RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...

journal_title：Plant molecular biology

authors： Tseng CC,Lee CJ,Chung YT,Sung TY,Hsieh MH

更新日期：2013-07-01 00:00:00

abstract：:Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...

journal_title：Plant molecular biology

authors： Gothandam KM,Nalini E,Karthikeyan S,Shin JS

更新日期：2010-01-01 00:00:00

abstract：:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

journal_title：Plant molecular biology

authors： Morris BA,Richardson KA,Andersen MT,Gardner RC

更新日期：1988-11-01 00:00:00

abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...

journal_title：Plant molecular biology

authors： Müller BS,Sakamoto T,de Menezes IP,Prado GS,Martins WS,Brondani C,de Barros EG,Vianello RP

更新日期：2014-11-01 00:00:00

abstract：:Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...

journal_title：Plant molecular biology

authors： Manninen I,Schulman AH

更新日期：1993-08-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:Lysine residue 9 of histone H3 can either be acetylated or mono-, di-, or tri-methylated. These epigenetic states have a diverse impact on regulating gene transcriptional activity and chromatin organization. H3K9ac is invariably correlated with transcriptional activation, whereas H3K9me2 has been reported to be mainly...

journal_title：Plant molecular biology

authors： Zhou J,Wang X,He K,Charron JB,Elling AA,Deng XW

更新日期：2010-04-01 00:00:00

abstract：:We have characterized the topoisomerase I and II activities in nuclear extracts from immature embryos of Zea mays and the effect of the treatment with 2,4-dichlorophenoxyacetic acid (2,4-D) and abscisic acid (ABA). These extracts were shown to be essentially devoid of protease and nuclease activities and they were tes...

journal_title：Plant molecular biology

authors： Carballo M,Giné R,Santos M,Puigdomènech P

更新日期：1991-01-01 00:00:00

abstract：:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

journal_title：Plant molecular biology

authors： Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

更新日期：2011-04-01 00:00:00

abstract：:The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...

journal_title：Plant molecular biology

authors： Smith CJ,Watson CF,Morris PC,Bird CR,Seymour GB,Gray JE,Arnold C,Tucker GA,Schuch W,Harding S

更新日期：1990-03-01 00:00:00

abstract：:A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...

journal_title：Plant molecular biology

authors： Karim S,Alezzawi M,Garcia-Petit C,Solymosi K,Khan NZ,Lindquist E,Dahl P,Hohmann S,Aronsson H

更新日期：2014-04-01 00:00:00

abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

journal_title：Plant molecular biology

authors： Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

更新日期：2009-05-01 00:00:00

abstract：:Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...

journal_title：Plant molecular biology

authors： Chapman KA,Delauney AJ,Kim JH,Verma DP

更新日期：1994-01-01 00:00:00

abstract：:Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...

journal_title：Plant molecular biology

authors： Asakura Y,Hagino T,Ohta Y,Aoki K,Yonekura-Sakakibara K,Deji A,Yamaya T,Sugiyama T,Sakakibara H

更新日期：2003-05-01 00:00:00

abstract：:14-3-3 proteins form a family of highly conserved proteins with central roles in many eukaryotic signalling networks. In plants, they bind to and activate the plasma membrane H+-ATPase, creating a binding site for the phytotoxin fusicoccin. Barley 14-3-3 transcripts accumulate in the epidermis upon inoculation with th...

journal_title：Plant molecular biology

authors： Finni C,Andersen CH,Borch J,Gjetting S,Christensen AB,de Boer AH,Thordal-Christensen H,Collinge DB

更新日期：2002-05-01 00:00:00

abstract：:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...

journal_title：Plant molecular biology

authors： Gupta R,Webster CI,Gray JC

更新日期：1998-04-01 00:00:00

abstract：:As derived from a cDNA clone, the structure of the b-32 protein of Zea mays, a putative regulatory factor of zein expression, has a central acidic region separated by two domains covered by secondary structure motifs. In this work, three b-32 genomic clones were selected from two genomic libraries obtained from the ma...

journal_title：Plant molecular biology

authors： Hartings H,Lazzaroni N,Marsan PA,Aragay A,Thompson R,Salamini F,Di Fonzo N,Palau J,Motto M

更新日期：1990-06-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...

journal_title：Plant molecular biology

authors： Roell MK,Morse DE

更新日期：1993-01-01 00:00:00

abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...

journal_title：Plant molecular biology

authors： Wang W,Takezawa D,Poovaiah BW

更新日期：1996-06-01 00:00:00

abstract：:In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...

journal_title：Plant molecular biology

authors： Stenzel I,Hause B,Miersch O,Kurz T,Maucher H,Weichert H,Ziegler J,Feussner I,Wasternack C

更新日期：2003-04-01 00:00:00

abstract：:We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...

journal_title：Plant molecular biology

authors： Rathore KS,Chowdhury VK,Hodges TK

更新日期：1993-03-01 00:00:00

abstract：:Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...

journal_title：Plant molecular biology

authors： Gong Z,Yamazaki M,Sugiyama M,Tanaka Y,Saito K

更新日期：1997-12-01 00:00:00

abstract：:Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...

journal_title：Plant molecular biology

authors： van der Zaal EJ,Memelink J,Mennes AM,Quint A,Libbenga KR

更新日期：1987-03-01 00:00:00

abstract：:Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...

journal_title：Plant molecular biology

authors： Ceccoli RD,Blanco NE,Medina M,Carrillo N

更新日期：2011-08-01 00:00:00

abstract：KEY MESSAGE:Modification of the poplar defense pathway through pathogen-induced expression of an amphibian host defense peptide modulates plant innate immunity and confers robust and reliable resistance against a major poplar pathogen, Septoria musiva. Host defense peptides (HDPs), also known as cationic antimicrobial ...

journal_title：Plant molecular biology

authors： Yevtushenko DP,Misra S

更新日期：2019-07-01 00:00:00

abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

journal_title：Plant molecular biology

authors： Hsieh MH,Chang CY,Hsu SJ,Chen JJ

更新日期：2008-04-01 00:00:00

abstract：:Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

journal_title：Plant molecular biology

authors： Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

更新日期：2009-02-01 00:00:00

abstract：:Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

journal_title：Plant molecular biology

authors： Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

更新日期：2010-12-01 00:00:00

abstract：:A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...

journal_title：Plant molecular biology

authors： Ohmido N,Akiyama Y,Fukui K

更新日期：1998-12-01 00:00:00