Constitutive and light-induced DNAseI hypersensitive sites in the rbcS genes of pea (Pisum sativum).

abstract：:DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...

journal_title：Plant molecular biology

authors： Prabhu RR,Gresshoff PM

更新日期：1994-10-01 00:00:00

abstract：:We describe a method for identifying signal transducing proteins from other organisms by their ability to turn on a signalling pathway when they are expressed at high level in yeast. The method was tested on a cDNA library from Arabidopsis thaliana, which was screened for clones that can activate glucose repression in...

journal_title：Plant molecular biology

authors： Thelander M,Fredriksson D,Schouten J,Hoge JH,Ronne H

更新日期：2002-05-01 00:00:00

abstract：:A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...

journal_title：Plant molecular biology

authors： Ohmido N,Akiyama Y,Fukui K

更新日期：1998-12-01 00:00:00

abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

journal_title：Plant molecular biology

authors： Riccardi G,De Rossi E,Della Valle G,Ciferri O

更新日期：1985-03-01 00:00:00

abstract：:The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...

journal_title：Plant molecular biology

authors： Cheng SH,Keller B,Condit CM

更新日期：1996-04-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...

journal_title：Plant molecular biology

authors： Putnam-Evans C,Wu J,Bricker TM

更新日期：1996-12-01 00:00:00

abstract：:The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...

journal_title：Plant molecular biology

authors： Pilgrim ML,Caspar T,Quail PH,McClung CR

更新日期：1993-10-01 00:00:00

abstract：:The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...

journal_title：Plant molecular biology

authors： Smigocki AC

更新日期：1991-01-01 00:00:00

abstract：:Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...

journal_title：Plant molecular biology

authors： Rubio V,Bustos R,Irigoyen ML,Cardona-López X,Rojas-Triana M,Paz-Ares J

更新日期：2009-03-01 00:00:00

abstract：:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three spec...

journal_title：Plant molecular biology

authors： Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe T

更新日期：2001-02-01 00:00:00

abstract：:We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...

journal_title：Plant molecular biology

authors： Roche D,Temple SJ,Sengupta-Gopalan C

更新日期：1993-09-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

journal_title：Plant molecular biology

authors： Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

更新日期：2003-01-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...

journal_title：Plant molecular biology

authors： Li ZK,Fu BY,Gao YM,Xu JL,Ali J,Lafitte HR,Jiang YZ,Rey JD,Vijayakumar CH,Maghirang R,Zheng TQ,Zhu LH

更新日期：2005-09-01 00:00:00

abstract：:ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...

journal_title：Plant molecular biology

authors： Crouzet J,Roland J,Peeters E,Trombik T,Ducos E,Nader J,Boutry M

更新日期：2013-05-01 00:00:00

abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

journal_title：Plant molecular biology

authors： Ni Z,Hu Z,Jiang Q,Zhang H

更新日期：2013-05-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...

journal_title：Plant molecular biology

authors： Tang W,Charles TM,Newton RJ

更新日期：2005-11-01 00:00:00

abstract：:The promoter region (-309 to +44) of the Brassica napus storage protein gene napA was studied in transgenic tobacco by successive 5' as well as internal deletions fused to the reporter gene GUS (beta-glucuronidase). The expression in the two main tissues of the seed, the endosperm and the embryo, was shown to be diffe...

journal_title：Plant molecular biology

authors： Ellerström M,Stålberg K,Ezcurra I,Rask L

更新日期：1996-12-01 00:00:00

abstract：KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...

journal_title：Plant molecular biology

authors： Geilfus CM,Lan J,Carpentier S

更新日期：2018-12-01 00:00:00

abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...

journal_title：Plant molecular biology

authors： Ciarbelli AR,Ciolfi A,Salvucci S,Ruzza V,Possenti M,Carabelli M,Fruscalzo A,Sessa G,Morelli G,Ruberti I

更新日期：2008-11-01 00:00:00

abstract：:Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...

journal_title：Plant molecular biology

authors： Koo JC,Chun HJ,Park HC,Kim MC,Koo YD,Koo SC,Ok HM,Park SJ,Lee SH,Yun DJ,Lim CO,Bahk JD,Lee SY,Cho MJ

更新日期：2002-10-01 00:00:00

abstract：:Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...

journal_title：Plant molecular biology

authors： Wang HJ,Wan AR,Hsu CM,Lee KW,Yu SM,Jauh GY

更新日期：2007-03-01 00:00:00

abstract：:Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...

journal_title：Plant molecular biology

authors： Gachon C,Baltz R,Saindrenan P

更新日期：2004-01-01 00:00:00

abstract：:Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several...

journal_title：Plant molecular biology

authors： Kersten B,Feilner T,Kramer A,Wehrmeyer S,Possling A,Witt I,Zanor MI,Stracke R,Lueking A,Kreutzberger J,Lehrach H,Cahilll DJ

更新日期：2003-07-01 00:00:00

abstract：:Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...

journal_title：Plant molecular biology

authors： Chaal BK,Ishida K,Green BR

更新日期：2003-05-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...

journal_title：Plant molecular biology

authors： Wu TM,Lin KC,Liau WS,Chao YY,Yang LH,Chen SY,Lu CA,Hong CY

更新日期：2016-01-01 00:00:00

abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...

journal_title：Plant molecular biology

authors： Gepstein S,Glick BR

更新日期：2013-08-01 00:00:00