Abstract:
:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; however, useful and reliable fluorescent organelle marker lines are lacking in the monocot model rice. Here, we developed eight different GFP-based organelle markers in transgenic rice and created a set of DsRed-based gateway vectors for combining with the marker lines. Two mitochondrial-localized rice ascorbate peroxidase genes fused to DsRed and successfully co-localized with mitochondrial-targeted marker lines verified the practical use of this system. The co-localization of GFP-fusion marker lines and DsRed-fusion proteins provide a convenient platform for in vivo or in vitro analysis of subcellular localization of rice proteins.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Wu TM,Lin KC,Liau WS,Chao YY,Yang LH,Chen SY,Lu CA,Hong CYdoi
10.1007/s11103-015-0397-8subject
Has Abstractpub_date
2016-01-01 00:00:00pages
107-15issue
1-2eissn
0167-4412issn
1573-5028pii
10.1007/s11103-015-0397-8journal_volume
90pub_type
杂志文章abstract::The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023606
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014491
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000036368.74758.66
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journal_title:Plant molecular biology
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更新日期:1998-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9786-9
更新日期:2011-08-01 00:00:00
abstract::The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006355510883
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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