Plant aldolase: cDNA and deduced amino-acid sequences of the chloroplast and cytosol enzyme from spinach.

Abstract:

:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the two enzymes from spinach share only 54% homology on their amino acid level whereas the homology of the cytosolic enzyme of spinach with the known sequences of cytosolic aldolases of maize, rice and Arabidopsis range from 67 to 92%. The sequence of the chloroplastic enzyme includes a stroma-targeting N-terminal transit peptide of 46 amino acid residues for import into the chloroplast. The transit peptide exhibits essential features similar to other chloroplast transit peptides. Southern blot analysis implies that both spinach enzymes are encoded by single genes.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Pelzer-Reith B,Penger A,Schnarrenberger C

doi

10.1007/BF00019948

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

331-40

issue

2

eissn

0167-4412

issn

1573-5028

journal_volume

21

pub_type

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