Abstract:
:The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolated and characterized the corresponding clone, Merihb1, from Arabidopsis. Analysis shows that the predicted MERIHB1 protein exhibits strong homology to KN1 and RS1 from maize, SBH1 from soybean, and KNAT1 and KNAT2 from Arabidopsis. Merihb1 is highly expressed in mRNA from cauliflower meristems and also accumulates in stem and flower mRNA. Based on the similarity of the Merihb1 and Kn1 sequences, expression patterns, and in situ hybridizations, we suggest that Merihb1 represents an Arabidopsis homologue of the maize Kn1 gene.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Granger CL,Callos JD,Medford JIdoi
10.1007/BF00021797subject
Has Abstractpub_date
1996-05-01 00:00:00pages
373-8issue
2eissn
0167-4412issn
1573-5028journal_volume
31pub_type
杂志文章abstract::The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1014091032750
更新日期:2002-03-01 00:00:00
abstract::We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3249-5
更新日期:2004-09-01 00:00:00
abstract::In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019952
更新日期:1993-01-01 00:00:00
abstract::The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019093
更新日期:1996-11-01 00:00:00
abstract::In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4922-z
更新日期:2006-03-01 00:00:00
abstract::RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0068-6
更新日期:2013-07-01 00:00:00
abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005790927581
更新日期:1997-03-01 00:00:00
abstract::The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029654
更新日期:1984-07-01 00:00:00
abstract::A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006062905742
更新日期:1998-12-01 00:00:00
abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019121
更新日期:1995-10-01 00:00:00
abstract::WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0063-y
更新日期:2013-07-01 00:00:00
abstract::Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042047
更新日期:1995-04-01 00:00:00
abstract::Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000028730.10834.e3
更新日期:2004-01-01 00:00:00
abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9448-8
更新日期:2009-04-01 00:00:00
abstract::We have identified 39 Arabidopsis thaliana ESTs encoding germin-like proteins (GLPs) and have completely sequenced 25 of these cDNAs. Our analysis demonstrates that the Arabidopsis genome contains a gene family with at least 12 GLP genes. Comparisons with other known germins and germin-like proteins indicate that thes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006038117130
更新日期:1998-12-01 00:00:00
abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0076-6
更新日期:2013-09-01 00:00:00
abstract:KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-00819-1
更新日期:2019-03-01 00:00:00
abstract:KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0707-z
更新日期:2018-03-01 00:00:00
abstract::Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000028775.58537.fe
更新日期:2004-01-01 00:00:00
abstract::Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9099-6
更新日期:2007-02-01 00:00:00
abstract::Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028983
更新日期:1993-09-01 00:00:00
abstract::The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9226-z
更新日期:2007-11-01 00:00:00
abstract::ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0053-0
更新日期:2013-05-01 00:00:00
abstract::The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418259
更新日期:1985-11-01 00:00:00
abstract::Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1025424814739
更新日期:2003-07-01 00:00:00
abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
abstract::Tandemly arrayed genes (TAGs) account for about one-third of the duplicated genes in eukaryotic genomes. They provide raw genetic material for biological evolution, and play important roles in genome evolution. The 22-kDa prolamin genes in cereal genomes represent typical TAG organization, and provide the good materia...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9705-5
更新日期:2010-12-01 00:00:00
abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1005816823636
更新日期:1997-12-01 00:00:00
abstract::In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00033603
更新日期:1988-11-01 00:00:00