Abstract:
:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that includes a hydrophobic region. Approximately 30 paralogues were identified in the Arabidopsis genome. Proteins with PPC-like domains are found in Bacteria, Archaea and the plant kingdom, but in Bacteria and Archaea the PPC containing proteins of do not have an AT-hook motif. Thus, the PPC domain is evolutionary conserved and has a new function such as AT-rich DNA binding. AHL1 was mainly localized in the nucleoplasm, but little in the nucleolus and heterochromatic region, and was concentrated in the boundary region between euchromatin and heterochromatin. Biochemically, AHL1 was also found in the nuclear matrix fraction. In the M phase, AHL1 was localized on the chromosomal surface. The AT-hook motif was essential for matrix attachment region (MAR) binding, and the hydrophobic region of the PPC was indispensable for nuclear localization. Our results suggest that AHL1 is a novel plant MAR binding protein, which is related to the positioning of chromatin fibers in the nucleus by the presence of an AT-hook motif and PPC domain. In addition, AHL1 is located on the surface of chromosomes during mitosis.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Fujimoto S,Matsunaga S,Yonemura M,Uchiyama S,Azuma T,Fukui Kdoi
10.1007/s11103-004-3249-5subject
Has Abstractpub_date
2004-09-01 00:00:00pages
225-39issue
2eissn
0167-4412issn
1573-5028pii
DO00003249journal_volume
56pub_type
杂志文章abstract::Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...
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journal_title:Plant molecular biology
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更新日期:1998-03-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1990-06-01 00:00:00
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更新日期:1999-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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更新日期:2010-05-01 00:00:00