Abstract：
:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were generated and used to inoculate tobacco plants lacking the N resistance gene (genotype Xanthi nn). Approximately 1/1000 of the infectious transcripts produced local lesions, and may thus elicit the HR. The cDNA inserts from 50 lesion-forming clones were recovered by RT-PCR, and 12 unique clones were sequenced. Comparisons with protein databases revealed homologies to (a) ubiquitin, (b) tobacco tumor-related protein, similar to Kunitz-type trypsin inhibitors and (c) ribosomal protein S14. The remaining nine clones revealed no homology to known proteins and are thus considered novel. Five clones were able to induce the expression of PR2, a gene which is specifically activated in the tobacco HR. Northern and western blot analyses of leaves infected by the clone encoding ubiquitin strongly suggest that the infection produced a co-suppression response; the endogenous level of ubiquitin mRNA and protein in infected leaves are ca. 50% less than those found in healthy leaves. This observation supports a previous report on the involvement of the ubiquitin system in the tobacco HR [2], and validates and utility of the functional cloning method.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Karrer EE,Beachy RN,Holt CAdoi
10.1023/a:1005949304445subject
Has Abstractpub_date
1998-03-01 00:00:00pages
681-90issue
5eissn
0167-4412issn
1573-5028journal_volume
36pub_type
杂志文章abstract：:We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028815
更新日期：1993-02-01 00:00:00
abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020195
更新日期：1995-01-01 00:00:00
abstract：:DNA sequence, copy number, expression and phylogenetic relevance of the psbA gene from the abundant marine prokaryote P. marinus CCMP 1375 was analyzed. The 7 amino acids near the C-terminus missing in higher plant and in Prochlorothrix hollandica D1 proteins are present in the derived amino acid sequence. P. marinus ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020892
更新日期：1995-03-01 00:00:00
abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9296-6
更新日期：2008-04-01 00:00:00
abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014935
更新日期：1993-05-01 00:00:00
abstract：:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039553
更新日期：1994-10-01 00:00:00
abstract：:We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017817
更新日期：1996-01-01 00:00:00
abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9229-9
更新日期：2007-11-01 00:00:00
abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578377
更新日期：1983-07-01 00:00:00
abstract：:Arabidopsis accessions are either winter-annuals, which require cold winter temperatures for spring flowering, or rapid-cycling summer annuals. Typically, winter annual accessions have functional FRIGIDA (FRI) and FRIGIDA-LIKE 1 (FRL1) proteins that promote high expression of FLOWERING LOCUS C (FLC), which prevents fl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9635-2
更新日期：2010-07-01 00:00:00
abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037051
更新日期：1991-10-01 00:00:00
abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006031822141
更新日期：1998-07-01 00:00:00
abstract：:Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006204318499
更新日期：1999-07-01 00:00:00
abstract：:We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028970
更新日期：1993-09-01 00:00:00
abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019182
更新日期：1995-01-01 00:00:00
abstract：:We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014494
更新日期：1992-10-01 00:00:00
abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020122
更新日期：1996-01-01 00:00:00
abstract：KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00870-6
更新日期：2019-07-01 00:00:00
abstract：:The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/b:plan.0000004333.96451.11
更新日期：2003-08-01 00:00:00
abstract：:Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0087-3
更新日期：2013-11-01 00:00:00
abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2525-8
更新日期：2004-09-01 00:00:00
abstract：:In this study, we report the cloning of the SsCut gene encoding cutinase from Sclerotinia sclerotiorum. We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa. Heterologous expression of SsCut in Escherichia coli (His-SsCut) caused the formation of lesions in tobacco th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0244-3
更新日期：2014-11-01 00:00:00
abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00945-4
更新日期：2020-02-01 00:00:00
abstract：:The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006493021557
更新日期：2001-01-01 00:00:00
abstract：:Due to an unfortunate turn of events, the first name of the fifth author appeared incorrectly in the original publication and should have read Guangbing. The correct representation of the authors' names and their affiliation is listed here and should be treated as definitive. ...
journal_title：Plant molecular biology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s11103-018-0705-1
更新日期：2018-02-01 00:00:00
abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF02418760
更新日期：1985-03-01 00:00:00
abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006355510883
更新日期：2000-03-01 00:00:00
abstract：:In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0320-3
更新日期：2015-06-01 00:00:00
abstract：:We have isolated full-size cDNA sequences encoding the photosynthetic isoform of pyruvate orthophosphate dikinase (PPDK) of the C3 plant Flaveria pringlei. The encoded protein shares 96% identical amino acid residues with the C4 isoform of PPDK in the C4 species F. trinervia. The differing amino acid residues are even...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00013761
更新日期：1994-10-01 00:00:00
abstract：:A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016515
更新日期：1990-05-01 00:00:00
