Abstract:
:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in mature leaves, callus and pre-flowering plants. This pattern of expression is confirmed using transgenic Arabidopsis plants containing a UBC6 promoter-GUS gene fusion construct. These plants display GUS activity in mature anthers prior to dehiscence, in developing embryos, sepals and the style after pollination.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Watts FZ,Butt N,Layfield P,Machuka J,Burke JF,Moore ALdoi
10.1007/BF00039553subject
Has Abstractpub_date
1994-10-01 00:00:00pages
445-51issue
1eissn
0167-4412issn
1573-5028journal_volume
26pub_type
杂志文章abstract::The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-6268-6
更新日期:2006-05-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00
abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
abstract::Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9585-8
更新日期:2010-03-01 00:00:00
abstract::The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040578
更新日期:1994-01-01 00:00:00
abstract::The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019093
更新日期:1996-11-01 00:00:00
abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0691-8
更新日期:2018-02-01 00:00:00
abstract::Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9984-0
更新日期:2013-01-01 00:00:00
abstract::Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005904230755
更新日期:1998-03-01 00:00:00
abstract::Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029004
更新日期:1993-10-01 00:00:00
abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043873
更新日期:1994-06-01 00:00:00
abstract::Sugarcane (Saccharum spp. hybrids) is a major feedstock for commercial bioethanol production. The recent integration of conversion technologies that utilize lignocellulosic sugarcane residues as well as sucrose from stem internodes has elevated bioethanol yields. RNAi suppression of lignin biosynthetic enzymes is a su...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0527-y
更新日期:2016-11-01 00:00:00
abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021807
更新日期:1996-05-01 00:00:00
abstract::Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043663
更新日期:1995-10-01 00:00:00
abstract::An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418760
更新日期:1985-03-01 00:00:00
abstract::Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9956-4
更新日期:2012-11-01 00:00:00
abstract::Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00032663
更新日期:1995-09-01 00:00:00
abstract::Tandemly arrayed genes (TAGs) account for about one-third of the duplicated genes in eukaryotic genomes. They provide raw genetic material for biological evolution, and play important roles in genome evolution. The 22-kDa prolamin genes in cereal genomes represent typical TAG organization, and provide the good materia...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9705-5
更新日期:2010-12-01 00:00:00
abstract::Identification of downstream target genes of stress-relating transcription factors (TFs) is desirable in understanding cellular responses to various environmental stimuli. However, this has long been a difficult work for both experimental and computational practices. In this research, we presented a novel computationa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9414-5
更新日期:2009-01-01 00:00:00
abstract::A cold-regulated gene (cor tmc-ap3) coding for a putative chloroplastic amino acid selective channel protein was isolated from cold-treated barley leaves combining the differential display and the 5'-RACE techniques. Cor tmc-ap3 is expressed at low level under normal growing temperature, and its expression is strongly...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375332677
更新日期:1999-09-01 00:00:00
abstract::A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
abstract::Chimaeric PCaMV35Scry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cry IA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indic...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020398
更新日期:1995-06-01 00:00:00
abstract::The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0122-4
更新日期:2014-01-01 00:00:00
abstract::The variation in grain hardness is the single most important trait that determines end-use quality of wheat. Grain texture classification is based primarily on either the resistance of kernels to crushing or the particle size distribution of ground grain or flour. Recently, the molecular genetic basis of grain hardnes...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1014837431178
更新日期:2002-03-01 00:00:00
abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0320-3
更新日期:2015-06-01 00:00:00
abstract::Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006199003756
更新日期:1999-01-01 00:00:00
abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016123
更新日期:1990-11-01 00:00:00
abstract::We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000006936.50532.3a
更新日期:2003-10-01 00:00:00
abstract::The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010665831955
更新日期:2001-05-01 00:00:00
abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0555-7
更新日期:2017-02-01 00:00:00