Uncovering the post-embryonic role of embryo essential genes in Arabidopsis using the controlled induction of visibly marked genetic mosaics: EMB506, an illustration.

Abstract:

:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of EMB506 gene expression in Arabidopsis we have generated green fluorescent protein (GFP) marked emb506 mosaic sectors at temporally defined stages during embryogenesis and additionally during various stages of vegetative growth, in otherwise phenotypically wild-type plants. We confirm the essential requirement for EMB506 gene expression in chloroplast biogenesis as reflected by the decreased chlorophyll content in emb506 mosaic sectors. We also show that the influence of EMB506 gene expression as it impinges on chloroplast biogenesis is first relevant at an intermediate stage in embryogenesis and that the role of EMB506 gene expression in chloroplast biogenesis is distinct from the essential role of EMB506 gene expression during early embryo development. By inducing emb506 mosaicism after the essential requirement for EMB506 gene expression in embryogenesis and also during vegetative growth we reveal that EMB506 gene expression additionally is required for correct cotyledon-, true leaf- and cauline leaf margin development. The strategy that we describe can be tailored to the mosaic analysis of any cloned EMB gene for which a corresponding mutant exists and can be applied to the mosaic analysis of mutant lethal genes in general.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Latvala-Kilby SM,Kilby NJ

doi

10.1007/s11103-006-6268-6

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

179-94

issue

1-2

eissn

0167-4412

issn

1573-5028

journal_volume

61

pub_type

杂志文章
  • Cloning and characterization of a novel CBL-interacting protein kinase from maize.

    abstract::A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9445-y

    authors: Zhao J,Sun Z,Zheng J,Guo X,Dong Z,Huai J,Gou M,He J,Jin Y,Wang J,Wang G

    更新日期:2009-04-01 00:00:00

  • Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment.

    abstract::The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039024

    authors: Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

    更新日期:1988-07-01 00:00:00

  • Site-directed mutagenesis of a tetrameric dandelion polyphenol oxidase (PPO-6) reveals the site of subunit interaction.

    abstract::Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-012-9943-9

    authors: Dirks-Hofmeister ME,Inlow JK,Moerschbacher BM

    更新日期:2012-09-01 00:00:00

  • Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression.

    abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016071

    authors: van der Zaal EJ,Droog FN,Boot CJ,Hensgens LA,Hoge JH,Schilperoort RA,Libbenga KR

    更新日期:1991-06-01 00:00:00

  • Characterization of radish mitochondrial atpA: influence of nuclear background on transcription of atpA-associated sequences and relationship with male sterility.

    abstract::We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016123

    authors: Makaroff CA,Apel IJ,Palmer JD

    更新日期:1990-11-01 00:00:00

  • Senescence-induced expression of a homologue of delta 9 desaturase in rose petals.

    abstract::cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041154

    authors: Fukuchi-Mizutani M,Savin K,Cornish E,Tanaka Y,Ashikari T,Kusumi T,Murata N

    更新日期:1995-11-01 00:00:00

  • Intraspecific length heterogeneity of the rDNA-IGR in Arabidopsis thaliana due to homologous recombination.

    abstract::A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the o...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015983

    authors: Luschnig C,Bachmair A,Schweizer D

    更新日期:1993-06-01 00:00:00

  • Comparative genetics of flowering time.

    abstract::Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genet...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:

    authors: Laurie DA

    更新日期:1997-09-01 00:00:00

  • Vir-115 gene product is required to stabilize D1 translation intermediates in chloroplasts.

    abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00043874

    authors: Kim J,Klein PG,Mullet JE

    更新日期:1994-06-01 00:00:00

  • Inducible expression of p50 from TMV for increased resistance to bacterial crown gall disease in tobacco.

    abstract::The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0122-4

    authors: Niemeyer J,Ruhe J,Machens F,Stahl DJ,Hehl R

    更新日期:2014-01-01 00:00:00

  • Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation.

    abstract::Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9784-y

    authors: Poage M,Le Martret B,Jansen MA,Nugent GD,Dix PJ

    更新日期:2011-07-01 00:00:00

  • The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin.

    abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040644

    authors: Spence MJ,Henzl MT,Lammers PJ

    更新日期:1991-09-01 00:00:00

  • Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.

    abstract::A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00041395

    authors: Kim J,Leustek T

    更新日期:1996-12-01 00:00:00

  • Two ftsH-family genes encoded in the nuclear and chloroplast genomes of the primitive red alga Cyanidioschyzon merolae.

    abstract::The red algal chloroplast genome encodes an essential prokaryotic cell division gene, ftsH, which has never been found in the mitochondrial genome of any organism. To compare the conserved prokaryote-derived mechanism for mitochondrial division with that of chloroplasts, we cloned chloroplast- and nuclear-encoded ftsH...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006369104530

    authors: Itoh R,Takano H,Ohta N,Miyagishima S,Kuroiwa H,Kuroiwa T

    更新日期:1999-10-01 00:00:00

  • Temporally regulated expression of a yeast invertase in potato tubers allows dissection of the complex metabolic phenotype obtained following its constitutive expression.

    abstract::The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-2525-8

    authors: Junker BH,Wuttke R,Tiessen A,Geigenberger P,Sonnewald U,Willmitzer L,Fernie AR

    更新日期:2004-09-01 00:00:00

  • OsYSL18 is a rice iron(III)-deoxymugineic acid transporter specifically expressed in reproductive organs and phloem of lamina joints.

    abstract::Iron uptake and translocation in plants are important processes for both plant and human nutrition, whereas relatively little is known about the molecular mechanisms of iron transport within the plant body. Several reports have shown that yellow stripe 1 (YS1) and YS1-like (YSL) transporters mediate metal-phytosiderop...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-009-9500-3

    authors: Aoyama T,Kobayashi T,Takahashi M,Nagasaka S,Usuda K,Kakei Y,Ishimaru Y,Nakanishi H,Mori S,Nishizawa NK

    更新日期:2009-08-01 00:00:00

  • Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric viral DNA.

    abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019520

    authors: Morris BA,Richardson KA,Andersen MT,Gardner RC

    更新日期:1988-11-01 00:00:00

  • OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.

    abstract::Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9752-6

    authors: Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK

    更新日期:2011-04-01 00:00:00

  • Genetic integration of DORNRÖSCHEN and DORNRÖSCHEN-LIKE reveals hierarchical interactions in auxin signalling and patterning of the Arabidopsis apical embryo.

    abstract::DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-010-9721-5

    authors: Chandler JW,Cole M,Jacobs B,Comelli P,Werr W

    更新日期:2011-02-01 00:00:00

  • Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct.

    abstract::In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028867

    authors: Conceição Ada S,Van Vliet A,Krebbers E

    更新日期:1994-11-01 00:00:00

  • Stability of the maize chromosomal high-mobility-group proteins, HMGa and HMGb, in vivo.

    abstract::Chromosomal non-histone high-mobility-group (HMG) proteins represent essential components of eukaryotic chromatin and have also been isolated from a variety of plants. In maize, studies on structure and function of the two larger of the four major HMG proteins have recently been performed and are now extended by analy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00043885

    authors: Grasser KD,Hetz W,Feix G

    更新日期:1994-06-01 00:00:00

  • Characteristics of RNA silencing in plants: similarities and differences across kingdoms.

    abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/B:PLAN.0000028797.63168.a7

    authors: Susi P,Hohkuri M,Wahlroos T,Kilby NJ

    更新日期:2004-01-01 00:00:00

  • Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosum.

    abstract::Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017450

    authors: Thanh ND,Medgyesy P

    更新日期:1989-01-01 00:00:00

  • Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.

    abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9345-1

    authors: Lee MH,Kim B,Song SK,Heo JO,Yu NI,Lee SA,Kim M,Kim DG,Sohn SO,Lim CE,Chang KS,Lee MM,Lim J

    更新日期:2008-08-01 00:00:00

  • Information contents and dinucleotide compositions of plant intron sequences vary with evolutionary origin.

    abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/BF00040537

    authors: White O,Soderlund C,Shanmugan P,Fields C

    更新日期:1992-09-01 00:00:00

  • Transaldolase genes from the cyanobacteria Anabaena variabilis and Synechocystis sp. PCC 6803: comparison with other eubacterial and eukaryotic homologues.

    abstract::We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00017817

    authors: Köhler U,Cerff R,Brinkmann H

    更新日期:1996-01-01 00:00:00

  • Caught in action: fine-scale plastome evolution in the parasitic plants of Cuscuta section Ceratophorae (Convolvulaceae).

    abstract:KEY MESSAGE:An exhaustive analysis of a group of closely related parasitic plants shows a predominantly gradual reduction in plastid genome composition and provides the most reduced plastomes in the genus Cuscuta. Parasitic plants have a diminished to completely absent reliance on photosynthesis, and are characterized ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00884-0

    authors: Banerjee A,Stefanović S

    更新日期:2019-08-01 00:00:00

  • Field trial analysis of nitrate reductase co-suppression: a comparative study of 38 combinations of transgene loci.

    abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019126

    authors: Palauqui JC,Vaucheret H

    更新日期:1995-10-01 00:00:00

  • Expression of one of the members of the Arabidopsis chaperonin 60 beta gene family is developmentally regulated and wound-repressible.

    abstract::To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040585

    authors: Zabaleta E,Assad N,Oropeza A,Salerno G,Herrera-Estrella L

    更新日期:1994-01-01 00:00:00

  • Unusual inheritance of evolutionarily-related double-stranded RNAs in interspecific hybrid between rice plants Oryza sativa and Oryza rufipogon.

    abstract::Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006118304093

    authors: Moriyama H,Horiuchi H,Nitta T,Fukuhara T

    更新日期:1999-04-01 00:00:00