Abstract:
:The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was assessed by northern analysis of various tissues. Transcriptional activity was unimpaired by the mutation and, in contrast, a higher level of expression was obtained when compared to the wild-type. The cDNA sequence encoding the mutated gene was isolated by RT-PCR and compared to the wild-type gene. A single point mutation corresponding to the substitution of a G nucleotide by A was identified at position 1212 starting from the translation site. The alignment of the deduced amino acid sequences from the mutated and wild-type gene showed that this mutation resulted in the substitution of an Arg by a His residue at position 381. This Arg residue is a conserved amino acid for histidinol phosphate aminotransferase of many species. These results indicate that the identified mutation results in an altered histidinol phosphate aminotransferase enzyme that is unable to convert the substrate imidazole acetol phosphate to histidinol phosphate and thereby leads to the blockage of histidine biosynthesis. Possible consequences of this blockage on the expression of other amino acid biosynthesis genes were evaluated by analysing the expression of the dhdps gene encoding dihydrodipicolinate synthase, the first key enzyme of the lysine pathway.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
El Malki F,Jacobs Mdoi
10.1023/a:1006493021557subject
Has Abstractpub_date
2001-01-01 00:00:00pages
191-9issue
2eissn
0167-4412issn
1573-5028journal_volume
45pub_type
杂志文章abstract::The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037145
更新日期:1991-11-01 00:00:00
abstract::Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9456-3
更新日期:2009-05-01 00:00:00
abstract::A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF01595169
更新日期:1983-03-01 00:00:00
abstract::The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040578
更新日期:1994-01-01 00:00:00
abstract::Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-1004-1
更新日期:2005-11-01 00:00:00
abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037148
更新日期:1991-11-01 00:00:00
abstract::As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027403
更新日期:1988-05-01 00:00:00
abstract::Genes for histidyl-aspartyl (His-Asp) phosphorelay components (His-containing phosphotransfer proteins, HP, and response regulators, RR) were isolated from Zea mays L. to characterize their function in cytokinin signaling. Six type-A RRs (ZmRR1, ZmRR2, ZmRR4-ZmRR7), 3 type-B RRs (ZmRR8-ZmRR10), and 3 HPs (ZmHP1-ZmHP3)...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023971315108
更新日期:2003-05-01 00:00:00
abstract::More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II. One of these CTD-phosphatase-like (CPL) proteins, CPL2, dephosphorylates CTD-Ser5-PO4 in an intact RNA polymerase II complex and contains a double-stranded (ds)-RNA-...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9348-y
更新日期:2008-08-01 00:00:00
abstract::The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006355510883
更新日期:2000-03-01 00:00:00
abstract::The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9138-y
更新日期:2007-05-01 00:00:00
abstract::In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040726
更新日期:1996-08-01 00:00:00
abstract::The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042359
更新日期:1993-12-01 00:00:00
abstract::A method is described for the high frequency transformation of carrot proembryogenic suspension culture cells by a non-oncogenic Ti-plasmid vector (pGV3850::1103) which carried a chimaeric kanamycin resistance gene (nos-NPT-II). Plants were regenerated efficiently from transformed material by somatic embryogenesis in ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015034
更新日期:1987-05-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9281-5
更新日期:2008-03-01 00:00:00
abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019126
更新日期:1995-10-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9322-8
更新日期:2008-07-01 00:00:00
abstract::Two cDNAs encoding putative metallothionein (MT)-like peptides have been isolated from tomato (L. esculentum L.). The predicted protein products of these two cDNAs (LeMT(A) and LeMT(B)) are 72 and 83 amino acids respectively and both encode peptides with arrangements of cysteine residues characteristic of type II plan...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005769121822
更新日期:1997-02-01 00:00:00
abstract::Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029606
更新日期:1994-07-01 00:00:00
abstract::Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00039383
更新日期:1996-10-01 00:00:00
abstract::Ribulose bisphosphate carboxylase-oxygenase (Rubisco) is a key enzyme in the photosynthetic fixation of CO2 by the chloroplast. The synthesis of the enzyme is an example of the cooperation between the chloroplast and the nucleocytoplasmic compartments, as it is assembled from subunits encoded in the two respective gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021302
更新日期:1986-01-01 00:00:00
abstract::We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028815
更新日期:1993-02-01 00:00:00
abstract::In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021423
更新日期:1993-10-01 00:00:00
abstract::Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8395-x
更新日期:2005-08-01 00:00:00
abstract::Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006094015513
更新日期:1998-11-01 00:00:00
abstract::The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-6235-z
更新日期:2004-12-01 00:00:00
abstract::A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016515
更新日期:1990-05-01 00:00:00
abstract::The 22 kDa auxin-binding proteins in higher plants have received considerable attention as candidates for an auxin receptor. A cDNA clone Ca-ERabp1 of hot pepper (Capsicum annum) was isolated using the oligonucleotides as PCR primers. The cDNA codes for a polypeptide related to the major 22 kDa auxin-binding protein f...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020496
更新日期:1996-12-01 00:00:00
abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000019059.56489.ca
更新日期:2003-11-01 00:00:00