Abstract:
:We have previously shown that mRNA and protein encoded by the Pvlea-18 gene from Phaseolus vulgaris L., a member of a new family of late embryogenesis-abundant (LEA) proteins, accumulate in dark-grown bean seedlings not only in response to water deficit but also during optimal irrigation. In this work, we studied Pvlea-18 gene transcriptional regulation by using transgenic Arabidopsis thaliana plants containing a chimeric gene consisting of the Pvlea-18 promoter region and the 3'-nos terminator fused to the GUS gene-coding region. We demonstrate that the chimeric gene is active during Arabidopsis normal development under well-irrigated conditions, and that it is further induced in response to ABA and dehydration treatments. Replacing the 3'-nos terminator with the Pvlea-18 3' region led to an additional increase in expression during development and in response to dehydration, but not in response to exogenous ABA. These results reveal an enhancer effect of the Pvlea-18 3' region, which showed to be higher specifically under dehydration. The small decrease in Pvlea-18 promoter expression observed when transgenic plants treated with fluridone (an ABA biosynthesis inhibitor) were subjected to dehydration suggests that the Pvlea-18 gene dehydration response is predominantly ABA-independent. Finally, we present evidence indicating that Pvlea-18 gene expression is negatively regulated during etiolated growth, particularly in roots, in contrast to the expression pattern observed during normal development.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Moreno-Fonseca LP,Covarrubias AAdoi
10.1023/a:1010607223533subject
Has Abstractpub_date
2001-03-01 00:00:00pages
501-15issue
5eissn
0167-4412issn
1573-5028journal_volume
45pub_type
杂志文章abstract::Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006178710443
更新日期:1999-01-01 00:00:00
abstract::We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016069
更新日期:1991-06-01 00:00:00
abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00914-x
更新日期:2019-11-01 00:00:00
abstract::Two distinct cDNAs for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) were obtained from a glyphosate-tolerant tobacco cell line. The cDNAs were 89% identical and the predicted sequences of the mature proteins were greater than 83% identical with EPSPS proteins from other plants. Tobacco EPSPS proteins were more ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028730
更新日期:1991-12-01 00:00:00
abstract::The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021266320877
更新日期:2002-12-01 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
abstract::Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9511-0
更新日期:2009-09-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00
abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020626
更新日期:1985-09-01 00:00:00
abstract::The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-6268-6
更新日期:2006-05-01 00:00:00
abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9345-1
更新日期:2008-08-01 00:00:00
abstract:KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0516-1
更新日期:2016-10-01 00:00:00
abstract::In biosynthesis of octadecanoids and jasmonate (JA), the naturally occurring enantiomer is established in a step catalysed by the gene cloned recently from tomato as a single-copy gene (Ziegler et al., 2000). Based on sequence homology, four full-length cDNAs were isolated from Arabidopsis thaliana ecotype Columbia co...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023049319723
更新日期:2003-04-01 00:00:00
abstract::The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027380
更新日期:1993-08-01 00:00:00
abstract::Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040638
更新日期:1991-09-01 00:00:00
abstract::The nucleotide sequence and the 5' flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and the deduced amino-acid sequence has been compared to those of various photosynthetic and chemoautotrophic Eu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036916
更新日期:1990-08-01 00:00:00
abstract::The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9383-8
更新日期:2008-11-01 00:00:00
abstract::RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037063
更新日期:1991-10-01 00:00:00
abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0468-5
更新日期:2016-06-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract::Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9655-y
更新日期:2010-09-01 00:00:00
abstract::A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042240
更新日期:1996-06-01 00:00:00
abstract::The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011632927541
更新日期:2001-08-01 00:00:00
abstract::A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020388
更新日期:1995-06-01 00:00:00
abstract::A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023388
更新日期:1992-06-01 00:00:00
abstract::The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006030007333
更新日期:1998-08-01 00:00:00
abstract::We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028903
更新日期:1992-12-01 00:00:00
abstract::Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042230
更新日期:1996-06-01 00:00:00
abstract::Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019207
更新日期:1992-03-01 00:00:00
abstract::A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005897921567
更新日期:1997-12-01 00:00:00