Abstract:
:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-locus pattern. The number of alleles found per locus varied from 6 to 16. Combining the genetic variation observed for the characterized loci provides a unique genotype for all the individuals tested. Using intraspecific controlled crosses of Q. robur trees Mendelian inheritance could be shown for five loci.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl Jdoi
10.1023/a:1005736722794subject
Has Abstractpub_date
1997-04-01 00:00:00pages
1093-6issue
6eissn
0167-4412issn
1573-5028journal_volume
33pub_type
杂志文章abstract::Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9078-y
更新日期:2007-01-01 00:00:00
abstract::Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcriptio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9752-6
更新日期:2011-04-01 00:00:00
abstract::Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019389
更新日期:1990-06-01 00:00:00
abstract::Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9281-5
更新日期:2008-03-01 00:00:00
abstract::Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039430
更新日期:1990-12-01 00:00:00
abstract::APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9711-7
更新日期:2011-01-01 00:00:00
abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019864222515
更新日期:2002-10-01 00:00:00
abstract::The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006340308077
更新日期:2000-01-01 00:00:00
abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016071
更新日期:1991-06-01 00:00:00
abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0324-z
更新日期:2015-06-01 00:00:00
abstract::StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9462-5
更新日期:2009-05-01 00:00:00
abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020803
更新日期:1996-03-01 00:00:00
abstract::In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0640-6
更新日期:2017-09-01 00:00:00
abstract::The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005909703600
更新日期:1998-05-01 00:00:00
abstract:KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0542-z
更新日期:2016-12-01 00:00:00
abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005780529457
更新日期:1997-01-01 00:00:00
abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006487326533
更新日期:2001-01-01 00:00:00
abstract::Tree crops have a long juvenile period which is a serious constraint for genetic improvement and experimental research. For example, apple remains in a juvenile phase for more than five years after seed germination. Here, we report about induction of rapid flowering in apple seedlings using the Apple latent spherical ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9718-0
更新日期:2011-01-01 00:00:00
abstract:KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0794-x
更新日期:2018-12-01 00:00:00
abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020626
更新日期:1985-09-01 00:00:00
abstract::We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027365
更新日期:1993-08-01 00:00:00
abstract::Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0087-3
更新日期:2013-11-01 00:00:00
abstract::In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0397-8
更新日期:2016-01-01 00:00:00
abstract::The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0240-7
更新日期:2014-11-01 00:00:00
abstract::The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006464822434
更新日期:2000-09-01 00:00:00
abstract::In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021423
更新日期:1993-10-01 00:00:00
abstract::A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005765719873
更新日期:1997-05-01 00:00:00
abstract::We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014494
更新日期:1992-10-01 00:00:00
abstract::The interaction between a protein and DNA is involved in almost all cellular functions, and is vitally important in cellular processes. Two complementary approaches are used to detect the interactions between a transcription factor (TF) and DNA, i.e. the TF-centered or protein-DNA approach, and the gene-centered or DN...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0234-5
更新日期:2014-11-01 00:00:00