Identification and characterization of (GA/CT)n-microsatellite loci from Quercus petraea.

Abstract:

:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-locus pattern. The number of alleles found per locus varied from 6 to 16. Combining the genetic variation observed for the characterized loci provides a unique genotype for all the individuals tested. Using intraspecific controlled crosses of Q. robur trees Mendelian inheritance could be shown for five loci.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

doi

10.1023/a:1005736722794

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

1093-6

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

33

pub_type

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