Abstract:
:We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein Hdoi
10.1007/BF00039532subject
Has Abstractpub_date
1994-10-01 00:00:00pages
203-10issue
1eissn
0167-4412issn
1573-5028journal_volume
26pub_type
杂志文章abstract::The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017456
更新日期:1988-09-01 00:00:00
abstract::SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005875300606
更新日期:1997-07-01 00:00:00
abstract::The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019218
更新日期:1992-03-01 00:00:00
abstract::The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9239-7
更新日期:2007-12-01 00:00:00
abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005915507497
更新日期:1998-02-01 00:00:00
abstract::The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006296422485
更新日期:1999-08-01 00:00:00
abstract::The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-6268-6
更新日期:2006-05-01 00:00:00
abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019520
更新日期:1988-11-01 00:00:00
abstract::During the course of characterizing polymerase chain reaction products corresponding to protein kinases of a higher plant, Arabidopsis thaliana, we found a DNA fragment that potentially codes for a polypeptide with mosaic sequences of two classes of protein kinases, a tyrosine-specific and a serine/threonine-specific ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00046450
更新日期:1992-11-01 00:00:00
abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
更新日期:1993-09-01 00:00:00
abstract::A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020195
更新日期:1995-01-01 00:00:00
abstract::The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020616
更新日期:1996-04-01 00:00:00
abstract::14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021261614491
更新日期:2002-12-01 00:00:00
abstract::cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041154
更新日期:1995-11-01 00:00:00
abstract::Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011848528377
更新日期:2001-11-01 00:00:00
abstract::An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418760
更新日期:1985-03-01 00:00:00
abstract:KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00945-4
更新日期:2020-02-01 00:00:00
abstract::High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017873
更新日期:1985-01-01 00:00:00
abstract::A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006129703262
更新日期:1999-03-01 00:00:00
abstract::The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0465-8
更新日期:2016-06-01 00:00:00
abstract::When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020185
更新日期:1995-01-01 00:00:00
abstract::We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017731
更新日期:1990-07-01 00:00:00
abstract::The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0531-2
更新日期:2016-12-01 00:00:00
abstract::WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0063-y
更新日期:2013-07-01 00:00:00
abstract::The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023557
更新日期:1994-02-01 00:00:00
abstract::Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005924502336
更新日期:1998-02-01 00:00:00
abstract:KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0677-6
更新日期:2017-12-01 00:00:00
abstract::A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006031822141
更新日期:1998-07-01 00:00:00
abstract::GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0403-1
更新日期:2016-01-01 00:00:00
abstract::Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017721
更新日期:1990-07-01 00:00:00