Tissue-specific expression of an oat 12S seed globulin gene in developing tobacco seeds: differential mRNA and protein accumulation.

Abstract:

:We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Schubert R,Panitz R,Manteuffel R,Nagy I,Wobus U,Bäumlein H

doi

10.1007/BF00039532

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

203-10

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

26

pub_type

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