Abstract:
:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein of predicted size at levels higher than that of the endogenous phyB by immunoblot analysis. Although these transgenic plants did not show any phenotype in the dark, enhancement of the phyB-dependent inhibition of hypocotyl elongation and reduction of the phytochrome A (phyA)-dependent inhibition were observed.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Sakamoto K,Nagatani Adoi
10.1007/BF00040726subject
Has Abstractpub_date
1996-08-01 00:00:00pages
1079-82issue
5eissn
0167-4412issn
1573-5028journal_volume
31pub_type
杂志文章abstract:KEY MESSAGE:Degradation of nitrogen-rich purines is tightly and oppositely regulated under drought and low nitrogen supply in bread wheat. Allantoin is a key target metabolite for improving nitrogen homeostasis under stress. The metabolite allantoin is an intermediate of the catabolism of purines (components of nucleot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00831-z
更新日期:2019-03-01 00:00:00
abstract::We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027072
更新日期:1992-08-01 00:00:00
abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9448-8
更新日期:2009-04-01 00:00:00
abstract::As part of a strategy to define differences in genome organization and expression between cytoplasmic male-sterile (CMS) and male-fertile (MF) sugar-beet mitochondria, cDNA libraries from both mitochondrial genotypes were constructed. Preliminary screening with ribosomal RNA gene probes identified candidate cDNA clone...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024201
更新日期:1994-04-01 00:00:00
abstract::Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0546-8
更新日期:2017-01-01 00:00:00
abstract::Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005912822671
更新日期:1998-01-01 00:00:00
abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021193
更新日期:1995-07-01 00:00:00
abstract::Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2%...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00013751
更新日期:1994-10-01 00:00:00
abstract::Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0187-8
更新日期:2014-06-01 00:00:00
abstract::In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021423
更新日期:1993-10-01 00:00:00
abstract::Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9585-8
更新日期:2010-03-01 00:00:00
abstract::The role of the cell wall hydrolase polygalacturonase (PG) during fruit ripening was investigated using novel mutant tomato lines in which expression of the PG gene has been down regulated by antisense RNA. Tomato plants were transformed with chimaeric genes designed to express anti-PG RNA constitutively. Thirteen tra...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028773
更新日期:1990-03-01 00:00:00
abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7820-x
更新日期:2005-02-01 00:00:00
abstract::The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037557
更新日期:1985-11-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00752901
更新日期:1986-07-01 00:00:00
abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005780529457
更新日期:1997-01-01 00:00:00
abstract::The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized protein that specificall...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0115-3
更新日期:2014-01-01 00:00:00
abstract::Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-1004-1
更新日期:2005-11-01 00:00:00
abstract::In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9785-x
更新日期:2011-08-01 00:00:00
abstract::Nicotine biosynthesis in tobacco (Nicotiana tabacum L.) is highly regulated by jasmonic acid (JA). Two nuclear loci, A and B (renamed NIC1 and NIC2) have been identified that mediate JA-inducible nicotine formation and total alkaloid accumulation. NIC2 was recently shown to be a cluster of seven genes encoding Apetala...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0116-2
更新日期:2014-01-01 00:00:00
abstract::Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017450
更新日期:1989-01-01 00:00:00
abstract::Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006499808317
更新日期:2000-06-01 00:00:00
abstract::We describe a non-LTR retrotransposon family,BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9542-6
更新日期:2009-12-01 00:00:00
abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019103
更新日期:1996-11-01 00:00:00
abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/B:PLAN.0000028797.63168.a7
更新日期:2004-01-01 00:00:00
abstract::Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00046460
更新日期:1992-11-01 00:00:00
abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
abstract::Hevea brasiliensis, being the only source of commercial natural rubber, is an extremely economically important crop. In an effort to facilitate biological, biochemical and molecular research in rubber biosynthesis, here we report the use of next-generation massively parallel sequencing technologies and de novo transcr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9811-z
更新日期:2011-10-01 00:00:00