Abstract:
:The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit at a low level. This fragment contains a 22 bp element (W1), which has been demonstrated to be important for seed-specific expression when coupled as a trimer to a heterologous TATA box (de Pater et al., Plant Cell 5:877-886, 1993). Here we show that deletion of W1 in the natural promoter context resulted in a strongly decreased level of gene expression. A 4 bp mutation of W1 reduced the expression of truncated derivatives of the Psl promoter. A single copy of W1 coupled to the TATA box of the CaMV 35S promoter directed low gene expression in seeds and leaves. Multimerization enhanced the expression in seeds up to 100-fold, to levels found with the Psl promoter, whereas the expression level in leaves remained low. These results demonstrate that the W1 element is an essential control element in the Psl promoter. When taken out of its natural context and multimerized, it is sufficient for high expression in seeds.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
de Pater S,Pham K,Klitsie I,Kijne Jdoi
10.1007/BF00019103subject
Has Abstractpub_date
1996-11-01 00:00:00pages
515-23issue
3eissn
0167-4412issn
1573-5028journal_volume
32pub_type
杂志文章abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-005-7703-9
更新日期:2006-04-01 00:00:00
abstract::To isolate genes which are expressed preferentially during embryogenesis, a Douglas-fir embryogenesis cDNA library was constructed and differentially screened with cDNA probes made with mRNA from developing and mature embryos, respectively. The cDNA clone PM 2.1 was isolated based on its abundance in developing seeds ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005888616748
更新日期:1997-07-01 00:00:00
abstract::Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006299821980
更新日期:1999-05-01 00:00:00
abstract::The root knot nematode resistance gene Mi in tomato has been mapped in the pericentromeric region of chromosome 6. With the objective of isolating Mi through a map-based cloning approach, we have previously identified and ordered into a high-resolution genetic linkage map a variety of tightly linked molecular markers....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021430
更新日期:1993-10-01 00:00:00
abstract::The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039024
更新日期:1988-07-01 00:00:00
abstract::High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017873
更新日期:1985-01-01 00:00:00
abstract::The nucleotide sequence and the 5' flanking region of the rbcL gene coding for the large subunit of ribulose bisphosphate-1,5-carboxylase/oxygenase of Pylaiella littoralis, a brown alga, has been determined and the deduced amino-acid sequence has been compared to those of various photosynthetic and chemoautotrophic Eu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036916
更新日期:1990-08-01 00:00:00
abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020199
更新日期:1996-11-01 00:00:00
abstract::A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027164
更新日期:1992-12-01 00:00:00
abstract::In higher eudicotyledonous angiosperms the floral organs are typically arranged in four different whorls, containing sepals, petals, stamens and carpels. According to the ABC model, the identity of these organs is specified by floral homeotic genes of class A, A+B, B+C and C, respectively. In contrast to the sepal and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1025070827979
更新日期:2003-07-01 00:00:00
abstract::Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5620-6
更新日期:2006-03-01 00:00:00
abstract::Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2619-y
更新日期:2005-12-01 00:00:00
abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9866-x
更新日期:2012-02-01 00:00:00
abstract::Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039627
更新日期:1993-01-01 00:00:00
abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00166458
更新日期:1987-05-01 00:00:00
abstract::Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1024822312038
更新日期:2003-06-01 00:00:00
abstract::The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015875
更新日期:1987-09-01 00:00:00
abstract::A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and functional characterisation of a dehydration responsive nuclear-targeted HDZip transcriptional regulator, CpHB-7. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-0023-x
更新日期:2006-06-01 00:00:00
abstract::Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043653
更新日期:1995-10-01 00:00:00
abstract::Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9814-9
更新日期:2011-11-01 00:00:00
abstract::Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006496308160
更新日期:2000-04-01 00:00:00
abstract::To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010614522706
更新日期:2001-07-01 00:00:00
abstract::Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interact...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9639-y
更新日期:2010-07-01 00:00:00
abstract::The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0395-x
更新日期:2016-01-01 00:00:00
abstract::The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029654
更新日期:1984-07-01 00:00:00
abstract::Two hevein-like peptides from the seed of Pharbitis nil, designated Pharbitis nil antimicrobial peptide 1 (Pn-AMP1) and Pn-AMP2, had been purified previously. Both exhibit potent in vitro antifungal activity against a broad spectrum of phytopathogenic fungi. We now report the isolation of two cDNA clones, designated p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1019864222515
更新日期:2002-10-01 00:00:00
abstract::APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9711-7
更新日期:2011-01-01 00:00:00
abstract::We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9284-2
更新日期:2008-03-01 00:00:00