Dihydrofolate reductase from Daucus carota cell suspension cultures: purification, molecular and kinetic characterization.

abstract：:In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expressi...

journal_title：Plant molecular biology

authors： Conceição Ada S,Van Vliet A,Krebbers E

更新日期：1994-11-01 00:00:00

abstract：:Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...

journal_title：Plant molecular biology

authors： Scherer GF

更新日期：2002-06-01 00:00:00

abstract：:Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

journal_title：Plant molecular biology

authors： Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

更新日期：1986-07-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

journal_title：Plant molecular biology

authors： Teo WL,Kumar P,Goh CJ,Swarup S

更新日期：2001-07-01 00:00:00

abstract：:This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...

journal_title：Plant molecular biology

authors： Bick JA,Neelam A,Smith E,Nelson SJ,Hall JL,Williams LE

更新日期：1998-10-01 00:00:00

abstract：:RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

journal_title：Plant molecular biology

authors： Petersen BO,Albrechtsen M

更新日期：2005-07-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:A new heterogeneity of the rDNA spacer of Arabidopsis thaliana, resulting from variation in copy number of the so-called 'C' repeat located downstream of the presumptive polymerase I promoter, is reported. Variation is shown to occur within and between ecotypes. PCR analysis and sequence comparison suggests that the o...

journal_title：Plant molecular biology

authors： Luschnig C,Bachmair A,Schweizer D

更新日期：1993-06-01 00:00:00

abstract：:The putative RNA helicase encoded by the Arabidopsis gene At1g32490 is a homolog of the yeast splicing RNA helicases Prp2 and Prp22. We isolated a temperature-sensitive allele (rsw12) of the gene in a screen for root radial swelling mutants. Plants containing this allele grown at the restrictive temperature showed wea...

journal_title：Plant molecular biology

authors： Howles PA,Gebbie LK,Collings DA,Varsani A,Broad RC,Ohms S,Birch RJ,Cork AH,Arioli T,Williamson RE

更新日期：2016-05-01 00:00:00

abstract：:Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2%...

journal_title：Plant molecular biology

authors： Chung YY,Kim SR,Finkel D,Yanofsky MF,An G

更新日期：1994-10-01 00:00:00

abstract：:We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a,...

journal_title：Plant molecular biology

authors： Saisho D,Nambara E,Naito S,Tsutsumi N,Hirai A,Nakazono M

更新日期：1997-11-01 00:00:00

abstract：:During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

journal_title：Plant molecular biology

authors： Ngo P,Ozga JA,Reinecke DM

更新日期：2002-07-01 00:00:00

abstract：:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...

journal_title：Plant molecular biology

authors： Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

更新日期：1990-04-01 00:00:00

abstract：:We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

journal_title：Plant molecular biology

authors： Makaroff CA,Apel IJ,Palmer JD

更新日期：1990-11-01 00:00:00

abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

journal_title：Plant molecular biology

authors： Temple SJ,Bagga S,Sengupta-Gopalan C

更新日期：1998-06-01 00:00:00

abstract：:Plant responses to abiotic stresses are coordinated by arrays of growth and developmental programs. Phytohormones such as abscisic acid (ABA) and indole-3-acetic acid (IAA) play critical roles in developmental progresses and environmental responses through complex signalling networks. However, crosstalk between the tw...

journal_title：Plant molecular biology

authors： Du H,Wu N,Chang Y,Li X,Xiao J,Xiong L

更新日期：2013-11-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

journal_title：Plant molecular biology

authors： Burow MD,Chen ZY,Mouton TM,Moroney JV

更新日期：1996-05-01 00:00:00

abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

journal_title：Plant molecular biology

authors： Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

更新日期：2019-03-01 00:00:00

abstract：:A novel CBL-interacting protein kinase (CIPK) gene, ZmCIPK16, was isolated from maize (Zea mays), which has been certified to have two copies in the genome. The ZmCIPK16 is strongly induced in maize seedlings by PEG, NaCl, ABA, dehydration, heat and drought, but not by cold. A yeast two-hybrid assay demonstrated that ...

journal_title：Plant molecular biology

authors： Zhao J,Sun Z,Zheng J,Guo X,Dong Z,Huai J,Gou M,He J,Jin Y,Wang J,Wang G

更新日期：2009-04-01 00:00:00

abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

journal_title：Plant molecular biology

authors： Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

更新日期：1996-12-01 00:00:00

abstract：:DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...

journal_title：Plant molecular biology

authors： Babinger P,Völkl R,Cakstina I,Maftei A,Schmitt R

更新日期：2007-02-01 00:00:00

abstract：:A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...

journal_title：Plant molecular biology

authors： Neininger A,Seith B,Hoch B,Mohr H

更新日期：1994-06-01 00:00:00

abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...

journal_title：Plant molecular biology

authors： Borroto KE,Dure L 3rd

更新日期：1986-03-01 00:00:00

abstract：:A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glu...

journal_title：Plant molecular biology

authors： Sakamoto A,Takeba G,Shibata D,Tanaka K

更新日期：1990-08-01 00:00:00

abstract：:In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...

journal_title：Plant molecular biology

authors： Sessa G,Fluhr R

更新日期：1995-12-01 00:00:00

abstract：:β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

journal_title：Plant molecular biology

authors： Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

更新日期：2012-02-01 00:00:00

abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...

journal_title：Plant molecular biology

authors： Xu H,Wang N,Liu J,Qu C,Wang Y,Jiang S,Lu N,Wang D,Zhang Z,Chen X

更新日期：2017-05-01 00:00:00

abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...

journal_title：Plant molecular biology

authors： Gil P,Green PJ

更新日期：1997-07-01 00:00:00