Abstract:
:A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by ethylene. DNA sequencing indicates that the two clones were derived from two closely related RNA species that are related to published plant peroxidase sequences. Southern analysis indicates that there are 1-5 copies in a haploid genome of a gene homologous to the cDNA clones. The deduced amino acid sequences are homologous with a tobacco (55% sequence identity), a horseradish (53%), a turnip (45%), and a potato (41%) peroxidase. The cloned sequences do not encode the 33 kDa peroxidase from which the original synthetic probe was been derived, but rather other putative peroxidases. An increase in the level of mRNA is evident by 3 hours after ethephon or ethylene treatment and plateaus by 15 hours.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Morgens PH,Callahan AM,Dunn LJ,Abeles FBdoi
10.1007/BF00016504subject
Has Abstractpub_date
1990-05-01 00:00:00pages
715-25issue
5eissn
0167-4412issn
1573-5028journal_volume
14pub_type
杂志文章abstract::An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029154
更新日期:1992-10-01 00:00:00
abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005909703600
更新日期:1998-05-01 00:00:00
abstract::The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023991
更新日期:1991-03-01 00:00:00
abstract:KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0808-8
更新日期:2019-01-01 00:00:00
abstract::Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014876409166
更新日期:2002-03-01 00:00:00
abstract::The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015875
更新日期:1987-09-01 00:00:00
abstract::Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0106-4
更新日期:2013-12-01 00:00:00
abstract::We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028903
更新日期:1992-12-01 00:00:00
abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006196530079
更新日期:1999-05-01 00:00:00
abstract::Nicotine biosynthesis in tobacco (Nicotiana tabacum L.) is highly regulated by jasmonic acid (JA). Two nuclear loci, A and B (renamed NIC1 and NIC2) have been identified that mediate JA-inducible nicotine formation and total alkaloid accumulation. NIC2 was recently shown to be a cluster of seven genes encoding Apetala...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0116-2
更新日期:2014-01-01 00:00:00
abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020254
更新日期:1995-05-01 00:00:00
abstract::Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006118304093
更新日期:1999-04-01 00:00:00
abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005915507497
更新日期:1998-02-01 00:00:00
abstract::Despite recent progress auxin signal transduction remains largely scetchy and enigmatic. A good body of evidence supports the notion that the ABP1 could be a functional receptor or part of a receptor, respectively, but this is not generally accepted. Evidence for other functional receptors is lacking, as is any clearc...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-06-01 00:00:00
abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
更新日期:1993-09-01 00:00:00
abstract::Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020093
更新日期:1985-03-01 00:00:00
abstract::Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039519
更新日期:1994-10-01 00:00:00
abstract::Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00039383
更新日期:1996-10-01 00:00:00
abstract::One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019182
更新日期:1995-01-01 00:00:00
abstract::Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
abstract::Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPht1;4 gene, encoding a phosphate transporter of PHT1 family, was isolated from Brassica napus. BnPht1;4 possesses the major characteristic of PHT1 high-affinity Pi transporters in plants, such as plasma-membrane localizati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0249-y
更新日期:2014-12-01 00:00:00
abstract::We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017817
更新日期:1996-01-01 00:00:00
abstract::We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000006936.50532.3a
更新日期:2003-10-01 00:00:00
abstract::Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9100-4
更新日期:2007-03-01 00:00:00
abstract::The shoot apical meristem of higher plants is characterized by a conserved pattern of cell division, the functional significance of which is unclear. Although a causal role for cell division frequency and orientation in morphogenesis has been suggested, supporting data are limited. An alternative interpretation laying...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-005-7703-9
更新日期:2006-04-01 00:00:00
abstract::Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021532
更新日期:1993-11-01 00:00:00
abstract::The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9379-4
更新日期:2008-11-01 00:00:00
abstract:KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00881-3
更新日期:2019-08-01 00:00:00
abstract::Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9956-4
更新日期:2012-11-01 00:00:00
abstract::Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7507-3
更新日期:2005-09-01 00:00:00