Abstract:
:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescens and Manduca sexta. The criteria used to modify both genes demonstrate that the removal of sequence motifs potentially resulting in premature polyadenylation and transcript instability causes increased insect resistance. The expression of a cryIC-cryIA(b) fusion resulting in protection against S. exigua, H. virescens and M. sexta demonstrates the potential of expressing translational fusions, not only to broaden the insect resistance of transgenic plants, but also to simultaneously employ different gene classes in resistance management strategies.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser Bdoi
10.1007/BF00039519subject
Has Abstractpub_date
1994-10-01 00:00:00pages
51-9issue
1eissn
0167-4412issn
1573-5028journal_volume
26pub_type
杂志文章abstract::The SELF PRUNING (SP) gene controls the regularity of the vegetative-reproductive switch along the compound shoot of tomato and thus conditions the 'determinate' (sp/sp) and 'indeterminate' (SP_) growth habits of the plant. SP is a developmental regulator which is homologous to CENTRORADIALIS (CEN) from Antirrhinum an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/b:plan.0000004333.96451.11
更新日期:2003-08-01 00:00:00
abstract::The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9494-x
更新日期:2009-07-01 00:00:00
abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020803
更新日期:1996-03-01 00:00:00
abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006487326533
更新日期:2001-01-01 00:00:00
abstract::Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015670
更新日期:1988-03-01 00:00:00
abstract::Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006138221874
更新日期:1999-03-01 00:00:00
abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00914-x
更新日期:2019-11-01 00:00:00
abstract::We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027495
更新日期:1990-04-01 00:00:00
abstract::Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1013335217744
更新日期:2002-02-01 00:00:00
abstract::The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017456
更新日期:1988-09-01 00:00:00
abstract::Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019323
更新日期:1995-02-01 00:00:00
abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00752901
更新日期:1986-07-01 00:00:00
abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01071-2
更新日期:2021-01-01 00:00:00
abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022324920316
更新日期:2003-03-01 00:00:00
abstract::The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0248-z
更新日期:2014-12-01 00:00:00
abstract::The Arabidopsis ga1 mutant has very low levels of endogenous, active gibberellins and thus has an extreme dwarf phenotype; application of GA3 induces stem elongation and flower development. To test the hypothesis that GA action in this system involves changes in gene expression, we have cloned mRNAs whose abundance ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023557
更新日期:1994-02-01 00:00:00
abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375123496
更新日期:1999-11-01 00:00:00
abstract::Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9078-y
更新日期:2007-01-01 00:00:00
abstract::Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028803
更新日期:1993-02-01 00:00:00
abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0468-5
更新日期:2016-06-01 00:00:00
abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00040537
更新日期:1992-09-01 00:00:00
abstract::We describe a non-LTR retrotransposon family,BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9542-6
更新日期:2009-12-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020244
更新日期:1995-05-01 00:00:00
abstract::β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9865-y
更新日期:2012-02-01 00:00:00
abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0555-7
更新日期:2017-02-01 00:00:00
abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-010-9599-2
更新日期:2010-05-01 00:00:00
abstract::The root endophytic fungus Piriformospora indica enhances plant adaptation to environmental stress based on general and non-specific plant species mechanisms. In the present study, we integrated the ionomics, metabolomics, and transcriptomics data to identify the genes and metabolic regulatory networks conferring salt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0461-z
更新日期:2016-04-01 00:00:00
abstract::Wheat genes are present in physically small, gene-rich regions, interspersed by gene-poor blocks of retrotransposon-like repetitive sequences. One of the largest gene-rich regions is present around fraction length (FL) 0.8 of the short arm of wheat homoeologous group 1 chromosomes and is called '1S0.8 region'. The obj...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1014876409166
更新日期:2002-03-01 00:00:00
abstract::ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0053-0
更新日期:2013-05-01 00:00:00
abstract::Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9578-7
更新日期:2010-03-01 00:00:00