Abstract：
:Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen maize cDNA clones can be used to detect elements of this variability. Similar levels of polymorphism are observed when genomic DNA is digested with any of a number of different restriction enzymes and probed with individual clones. When a clone is hybridized to genomic DNAs prepared from several different maize lines, a number of different alleles are often detected at a single locus. At the same time one clone can often detect more than one independently segregating locus by cross hybridization to related sequences at other loci. As expected these markers are inherited as simple codominant Mendelian alleles from one generation to the next and colinkage of these markers can be demonstrated in the progeny from a heterozygous parent. In similar studies with tomato, remarkably different results were found. Few RFPs were demonstrable among domestic Lycopersicon esculentum lines although a higher level of variability could be detected when comparing esculentum with its wild Lycopersicon relatives. These results are discussed in relation to the applied uses of RFPs in plant breeding as well as the inherent variability of different plant genomes.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Helentjaris T,King G,Slocum M,Siedenstrang C,Wegman Sdoi
10.1007/BF00020093subject
Has Abstractpub_date
1985-03-01 00:00:00pages
109-18issue
2eissn
0167-4412issn
1573-5028journal_volume
5pub_type
杂志文章abstract：:To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006487326533
更新日期：2001-01-01 00:00:00
abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0954-7
更新日期：2005-03-01 00:00:00
abstract：:We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1016067200148
更新日期：2002-09-01 00:00:00
abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015670
更新日期：1988-03-01 00:00:00
abstract：KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00870-6
更新日期：2019-07-01 00:00:00
abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023618
更新日期：1993-03-01 00:00:00
abstract：:The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9138-y
更新日期：2007-05-01 00:00:00
abstract：:We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Bot...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00026794
更新日期：1992-07-01 00:00:00
abstract：:The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005874404706
更新日期：1997-08-01 00:00:00
abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023238
更新日期：1994-05-01 00:00:00
abstract：:The expression of the mat-r locus (mat-r-nad1e-nad5III) was studied in wheat mitochondria. Transcription initiation sites were mapped by S1 protection, primer extension and capping experiments. Two different transcription initiation sites were found. One, non-canonical promoter of low expression level generates a tran...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006296422485
更新日期：1999-08-01 00:00:00
abstract：:Chimaeric PCaMV35Scry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cry IA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indic...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020398
更新日期：1995-06-01 00:00:00
abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:Beyond the rewards of plant genome analysis and gene identification, characterisation of protein activities, post-translational modifications and protein complex composition remains a challenge for plant biologists. Ideally, methods should allow rapid isolation of proteins from plant material achieving a high degree o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-0501-y
更新日期：2004-05-01 00:00:00
abstract：:Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019553
更新日期：1996-03-01 00:00:00
abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01051-6
更新日期：2020-11-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:The functional homologues of vertebrate natriuretic peptides (NPs), the plant natriuretic peptides (PNPs), are a novel class of peptidic hormones that signal via guanosine 3',5'-cyclic monophosphate (cGMP) and systemically affect plant salt and water balance and responses to biotrophic plant pathogens. Although there ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0465-8
更新日期：2016-06-01 00:00:00
abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0460-0
更新日期：2016-06-01 00:00:00
abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF01578377
更新日期：1983-07-01 00:00:00
abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1011848528377
更新日期：2001-11-01 00:00:00
abstract：:In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037027
更新日期：1995-03-01 00:00:00
abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005915507497
更新日期：1998-02-01 00:00:00
abstract：:The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027380
更新日期：1993-08-01 00:00:00
abstract：:Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9578-7
更新日期：2010-03-01 00:00:00
abstract：:We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028815
更新日期：1993-02-01 00:00:00
abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9698-0
更新日期：2010-12-01 00:00:00
abstract：:Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-008-9380-y
更新日期：2009-03-01 00:00:00
abstract：:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027070
更新日期：1992-08-01 00:00:00
abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-013-0038-z
更新日期：2013-08-01 00:00:00
