Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture.

abstract：:The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

journal_title：Plant molecular biology

authors： White O,Soderlund C,Shanmugan P,Fields C

更新日期：1992-09-01 00:00:00

abstract：:The sesame 2S albumin (2Salb) promoter was evaluated for its capacity to express the reporter gusA gene encoding β-glucuronidase in transgenic tobacco seeds relative to the soybean fad3C gene promoter element. Results revealed increased expression of gusA gene in tobacco seed tissue when driven by sesame 2S albumin pr...

journal_title：Plant molecular biology

authors： Bhunia RK,Chakraborty A,Kaur R,Gayatri T,Bhattacharyya J,Basu A,Maiti MK,Sen SK

更新日期：2014-11-01 00:00:00

abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

journal_title：Plant molecular biology

authors： Leone M,Zavallo D,Venturuzzi A,Asurmendi S

更新日期：2020-11-01 00:00:00

abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

journal_title：Plant molecular biology

authors： Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

更新日期：2009-05-01 00:00:00

abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...

journal_title：Plant molecular biology

authors： Greene EA,Erard M,Dedieu A,Barker DG

更新日期：1998-03-01 00:00:00

abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

journal_title：Plant molecular biology

authors： Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

更新日期：2011-08-01 00:00:00

abstract：:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...

journal_title：Plant molecular biology

authors： Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

更新日期：2006-05-01 00:00:00

abstract：:Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...

journal_title：Plant molecular biology

authors： Pons C,Martí C,Forment J,Crisosto CH,Dandekar AM,Granell A

更新日期：2016-11-01 00:00:00

abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

journal_title：Plant molecular biology

authors： Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

更新日期：2019-03-01 00:00:00

abstract：:Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...

journal_title：Plant molecular biology

authors： Griffor MC,Vodkin LO,Singh RJ,Hymowitz T

更新日期：1991-07-01 00:00:00

abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...

journal_title：Plant molecular biology

authors： Hsieh WL,Wolniak SM

更新日期：1998-05-01 00:00:00

abstract：:Plant S-adenosyl-L-methionine-dependent methyltransferases (SAM-Mtases) are the key enzymes in phenylpropanoid, flavonoid and many other metabolic pathways of biotechnological importance. Here we compiled the amino acid sequences of 56 SAM-Mtases from different plants and performed a computer analysis for the conserve...

journal_title：Plant molecular biology

authors： Joshi CP,Chiang VL

更新日期：1998-07-01 00:00:00

abstract：:We have cloned and sequenced a full-length cDNA from barley (Hordeum vulgare L.) seeds encoding the bifunctional α-amylase/subtilisin inhibitor (BASI). The nucleotide sequence predicts an open reading frame coding for a protein of 203 amino acids. The first 22 amino acids exhibit the sequence characteristic of a signa...

journal_title：Plant molecular biology

authors： Leah R,Mundy J

更新日期：1989-06-01 00:00:00

abstract：:The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...

journal_title：Plant molecular biology

authors： Bischoff F,Vahlkamp L,Molendijk A,Palme K

更新日期：2000-02-01 00:00:00

abstract：KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...

journal_title：Plant molecular biology

authors： Chu P,Wilson GM,Michael TP,Vaiciunas J,Honig J,Lam E

更新日期：2018-10-01 00:00:00

abstract：:We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a,...

journal_title：Plant molecular biology

authors： Saisho D,Nambara E,Naito S,Tsutsumi N,Hirai A,Nakazono M

更新日期：1997-11-01 00:00:00

abstract：:A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...

journal_title：Plant molecular biology

authors： van Engelen FA,de Jong AJ,Meijer EA,Kuil CW,Meyboom JK,Dirkse WG,Booij H,Hartog MV,Vandekerckhove J,de Vries SC

更新日期：1995-03-01 00:00:00

abstract：:Lectin receptor-like kinases (LecRLKs) are members of RLK family composed of lectin-like extracellular recognition domain, transmembrane domain and cytoplasmic kinase domain. LecRLKs are plasma membrane proteins believed to be involved in signal transduction. However, most of the members of the protein family even in ...

journal_title：Plant molecular biology

authors： Vaid N,Pandey P,Srivastava VK,Tuteja N

更新日期：2015-05-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...

journal_title：Plant molecular biology

authors： Waldie T,Hayward A,Beveridge CA

更新日期：2010-05-01 00:00:00

abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...

journal_title：Plant molecular biology

authors： Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo F

更新日期：2010-03-01 00:00:00

abstract：:We investigated the spatial and temporal expression patterns of two rice calcium-dependent protein kinases (CDPKs), OsCDPK2 and OSCDPK11, using isoform-specific antisera. Bands of the expected molecular sizes for OsCDPK2 (59 kDa) and OsCDPK11 (61 kDa) were detected on western blots. OsCDPK2 and OsCDPK11 mRNA and prote...

journal_title：Plant molecular biology

authors： Frattini M,Morello L,Breviario D

更新日期：1999-12-01 00:00:00

abstract：:Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

journal_title：Plant molecular biology

authors： Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

更新日期：1999-01-01 00:00:00

abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...

journal_title：Plant molecular biology

authors： Bagga S,Sutton D,Kemp JD,Sengupta-Gopalan C

更新日期：1992-09-01 00:00:00

abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...

journal_title：Plant molecular biology

authors： Temple SJ,Bagga S,Sengupta-Gopalan C

更新日期：1998-06-01 00:00:00

abstract：:In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...

journal_title：Plant molecular biology

authors： van der Geest AH,Hall TC

更新日期：1996-11-01 00:00:00

abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...

journal_title：Plant molecular biology

authors： Tang W,Sun J,Liu J,Liu F,Yan J,Gou X,Lu BR,Liu Y

更新日期：2014-11-01 00:00:00

abstract：:An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...

journal_title：Plant molecular biology

authors： Lopato S,Gattoni R,Fabini G,Stevenin J,Barta A

更新日期：1999-03-01 00:00:00

abstract：:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

journal_title：Plant molecular biology

authors： Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

更新日期：2011-05-01 00:00:00

abstract：KEY MESSAGE:AFP improved cryopreservation efficiency of potato (Solanum tuberosum cv. Superior) by regulating transcript levels of CBF1 and DHN1. However, the optimal AFP concentration required for strong induction of the genes was dependent on the type of vitrification solution to which the AFP was added: This finding...

journal_title：Plant molecular biology

authors： Seo JH,Naing AH,Jeon SM,Kim CK

更新日期：2018-07-01 00:00:00