MtENOD16 and 20 are members of a family of phytocyanin-related early nodulins.

Abstract:

:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence alignment have defined the encoded early nodulins as a distinct sub-family of phytocyanin-related proteins, although the absence of key ligands implies that they are unlikely to bind copper. Molecular modelling based on known phytocyanin structure has been used to predict the 3-dimensional conformation of the principle globular domain of MtENOD16/20. Additional structural features common to both early nodulin and phytocyanin precursors include an N-terminal transit peptide, a highly variable (hydroxy)proline-rich sequence which probably undergoes extensive post-translational modification, and a hydrophobic C-terminal tail.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Greene EA,Erard M,Dedieu A,Barker DG

doi

10.1023/a:1005916821224

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

775-83

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

36

pub_type

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