Abstract:
:The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by another phytohormone, abscisic acid. In situ hybridization experiments indicate that EPB is expressed in the scutellar epithelium within 24 h of seed germination, but the aleurone tissue surrounding the starchy endosperm eventually becomes the main tissue expressing this enzyme. The EPB gene family of barley consists of two very similar genes, EPB1 and EPB2, both of which have been mapped to chromosome 3. The sequences of EPB1 and EPB2 match with the two previously published cDNA clones indicating that both genes are expressed. Interestingly, neither of these genes contain any introns, a rare phenomenon in which all members of a small gene family are active intronless genes. Sequence comparison indicates that the barley EPB family can be classified as cathepsin L-like endopeptidases and is most closely related to two legume cysteine proteinases (Phaseolus vulgaris EP-C1 and Vigna mungo SHEP) which are also involved in seed storage protein degradation. The promoters of EPB1 and EPB2 have been linked to the coding sequence of a reporter gene, GUS, encoding beta-glucuronidase, and introduced into barley aleurone cells using the particle bombardment method. Transient expression studies indicate that EPB promoters are sufficient to confer the hormonal regulation of these genes.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Mikkonen A,Porali I,Cercos M,Ho THdoi
10.1007/BF00021787subject
Has Abstractpub_date
1996-05-01 00:00:00pages
239-54issue
2eissn
0167-4412issn
1573-5028journal_volume
31pub_type
杂志文章abstract::In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...
journal_title:Plant molecular biology
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pub_type: 杂志文章
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journal_title:Plant molecular biology
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pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0397-8
更新日期:2016-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2000-06-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1995-09-01 00:00:00
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更新日期:2019-05-01 00:00:00
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更新日期:1985-03-01 00:00:00
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更新日期:2010-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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更新日期:1997-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2002-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
abstract::Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...
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