Abstract：
:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show that oatPHYAox plants could be induced to set bud and become cold hardy by exposure to a shorter, non-24 h diurnal cycle that significantly alters the relative position between endogenous rhythms and perceived light/dark cycles. Furthermore, we describe studies in which the expression of endogenous Populus tremula x P. tremuloides PHYTOCHROME A (PttPHYA) was reduced in Populus trees by antisense inhibition. The antisense plants showed altered photoperiodic requirements, resulting in earlier growth cessation and bud formation in response to daylength shortening, an effect that was explained by an altered innate period that leads to phase changes of clock-associated genes such as PttCO2. Moreover, gene expression studies following far-red light pulses show a phyA-mediated repression of PttLHY1 and an induction of PttFKF1 and PttFT. We conclude that the level of PttPHYA expression strongly influences seasonally regulated growth in Populus and is central to co-ordination between internal clock-regulated rhythms and external light/dark cycles through its dual effect on the pace of clock rhythms and in light signaling.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson MEdoi
10.1007/s11103-010-9619-2subject
Has Abstractpub_date
2010-05-01 00:00:00pages
143-56issue
1-2eissn
0167-4412issn
1573-5028journal_volume
73pub_type
杂志文章abstract：:A full-length cDNA encoding the 206 amino acid open reading frame of a trypsin/chymotrypsin inhibitor abundant in the corms of giant taro (Alocasia macrorrhiza) was isolated. An internal fragment was cloned using degenerate primers corresponding to a region of the mature protein sequence and the 'rapid amplification o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020813
更新日期：1996-03-01 00:00:00
abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1019851913083
更新日期：2002-10-01 00:00:00
abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020486
更新日期：1996-12-01 00:00:00
abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0195-8
更新日期：2014-07-01 00:00:00
abstract：:The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010665910095
更新日期：2001-03-01 00:00:00
abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00981-5
更新日期：2020-05-01 00:00:00
abstract：:RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0068-6
更新日期：2013-07-01 00:00:00
abstract：:Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-1303-y
更新日期：2004-07-01 00:00:00
abstract：:In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1005816823636
更新日期：1997-12-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006030007333
更新日期：1998-08-01 00:00:00
abstract：:Leaf senescence is one of the key stages of plant leaf development. It is a highly complex but ordered process involving expression of large scale senescence associated genes, and its molecular mechanisms still remain unclear. By using suppression subtractive hybridization, 815 ESTs that are up-regulated at the onset ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9300-1
更新日期：2008-05-01 00:00:00
abstract：:As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027403
更新日期：1988-05-01 00:00:00
abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-013-0038-z
更新日期：2013-08-01 00:00:00
abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041405
更新日期：1996-12-01 00:00:00
abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041395
更新日期：1996-12-01 00:00:00
abstract：:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005924502336
更新日期：1998-02-01 00:00:00
abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005915507497
更新日期：1998-02-01 00:00:00
abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026507517182
更新日期：2000-12-01 00:00:00
abstract：:Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1023900100803
更新日期：2003-05-01 00:00:00
abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005909703600
更新日期：1998-05-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
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更新日期：1996-05-01 00:00:00
abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041164
更新日期：1995-11-01 00:00:00
abstract：:The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019395
更新日期：1990-06-01 00:00:00
abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-018-0771-4
更新日期：2018-09-01 00:00:00
abstract：:The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005928219895
更新日期：1998-04-01 00:00:00
abstract：:We describe the molecular and functional characterization of three closely related S-adenosyl-L-methionine synthetase (SAMS) isoenzymes from Catharanthus roseus (Madagascar periwinkle). The genes are differentially expressed in cell cultures during growth of the culture and after application of various stresses (elici...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005711720930
更新日期：1997-01-01 00:00:00
abstract：:Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9448-8
更新日期：2009-04-01 00:00:00
abstract：:Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040831
更新日期：1996-09-01 00:00:00
abstract：KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00914-x
更新日期：2019-11-01 00:00:00
abstract：:Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006099512706
更新日期：1998-06-01 00:00:00
