Isolation and characterization of full-length cDNA clones of the giant taro (Alocasia macrorrhiza) trypsin/chymotrypsin inhibitor.

abstract：:With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...

journal_title：Plant molecular biology

authors： Hirochika H,Guiderdoni E,An G,Hsing YI,Eun MY,Han CD,Upadhyaya N,Ramachandran S,Zhang Q,Pereira A,Sundaresan V,Leung H

更新日期：2004-02-01 00:00:00

abstract：:Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice m...

journal_title：Plant molecular biology

authors： Xiao Y,You S,Kong W,Tang Q,Bai W,Cai Y,Zheng H,Wang C,Jiang L,Wang C,Zhao Z,Wan J

更新日期：2019-11-01 00:00:00

abstract：:The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant...

journal_title：Plant molecular biology

authors： Malinowski R,Higgins R,Luo Y,Piper L,Nazir A,Bajwa VS,Clouse SD,Thompson PR,Stratmann JW

更新日期：2009-07-01 00:00:00

abstract：:More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II. One of these CTD-phosphatase-like (CPL) proteins, CPL2, dephosphorylates CTD-Ser5-PO4 in an intact RNA polymerase II complex and contains a double-stranded (ds)-RNA-...

journal_title：Plant molecular biology

authors： Ueda A,Li P,Feng Y,Vikram M,Kim S,Kang CH,Kang JS,Bahk JD,Lee SY,Fukuhara T,Staswick PE,Pepper AE,Koiwa H

更新日期：2008-08-01 00:00:00

abstract：:A vector has been designed that contains a truncated CaMV (cauliflower mosaic virus) 35S promoter fused to a receptor gene encoding beta-glucuronidase (GUS), placed adjacent to the left border sequence of an Agrobacterium vector. In potato plants transformed with this vector, different patterns of transcription were d...

journal_title：Plant molecular biology

authors： Goldsbrough A,Bevan M

更新日期：1991-02-01 00:00:00

abstract：:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in ...

journal_title：Plant molecular biology

authors： Watts FZ,Butt N,Layfield P,Machuka J,Burke JF,Moore AL

更新日期：1994-10-01 00:00:00

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00

abstract：:Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...

journal_title：Plant molecular biology

authors： Waldie T,Hayward A,Beveridge CA

更新日期：2010-05-01 00:00:00

abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...

journal_title：Plant molecular biology

authors： Bagga S,Sutton D,Kemp JD,Sengupta-Gopalan C

更新日期：1992-09-01 00:00:00

abstract：:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...

journal_title：Plant molecular biology

authors： Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

更新日期：1990-04-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:DORNRÖSCHEN (DRN) and DORNRÖSCHEN-LIKE (DRNL) encode AP2-domain transcription factors, which act redundantly in cotyledon organogenesis. A more detailed genetic study now integrates DRN and DRNL into the CUP-SHAPED COTYLEDON (CUC) regulatory network and places DRN and DRNL differentially within the auxin signalling ne...

journal_title：Plant molecular biology

authors： Chandler JW,Cole M,Jacobs B,Comelli P,Werr W

更新日期：2011-02-01 00:00:00

abstract：KEY MESSAGE:Salt stress triggers a simultaneous transcriptional repression and aquaporin internalization to modify root cell water conductivity. Plasma membrane intrinsic proteins (PIPs) are involved in the adjustment of plant water balance in response to changing environmental conditions. In this study, Arabidopsis wi...

journal_title：Plant molecular biology

authors： Pou A,Jeanguenin L,Milhiet T,Batoko H,Chaumont F,Hachez C

更新日期：2016-12-01 00:00:00

abstract：:Opium poppy latex contains a group of laticifer-specific, low-molecular-weight polypeptides called major latex proteins (MLPs). Here we describe a new member of the MLP gene family (gMLP 22) which shares 79.6% nucleotide and 84.6% amino acid sequence identity with previously isolated clones. DNA gel blot analysis indi...

journal_title：Plant molecular biology

authors： Nessler CL,Burnett RJ

更新日期：1992-11-01 00:00:00

abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...

journal_title：Plant molecular biology

authors： Petitot AS,Blein JP,Pugin A,Suty L

更新日期：1997-10-01 00:00:00

abstract：:The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

journal_title：Plant molecular biology

authors： Montoliu L,Rigau J,Puigdomènech P

更新日期：1990-01-01 00:00:00

abstract：:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...

journal_title：Plant molecular biology

authors： Fujimoto S,Matsunaga S,Yonemura M,Uchiyama S,Azuma T,Fukui K

更新日期：2004-09-01 00:00:00

abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...

journal_title：Plant molecular biology

authors： Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

更新日期：1990-12-01 00:00:00

abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...

journal_title：Plant molecular biology

authors： Ohtani T,Galili G,Wallace JC,Thompson GA,Larkins BA

更新日期：1991-01-01 00:00:00

abstract：:Live imaging during floral development revealed that expression of the DORNRÖSCHEN-LIKE (DRNL) gene encoding an AP2-like transcription factor, marks all organ founder cells. Transcription precedes the perception of auxin response maxima as measured by the DR5 reporter and is unaffected in early organogenesis, by mutat...

journal_title：Plant molecular biology

authors： Chandler JW,Jacobs B,Cole M,Comelli P,Werr W

更新日期：2011-05-01 00:00:00

abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

journal_title：Plant molecular biology

authors： Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

更新日期：1992-02-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：:A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...

journal_title：Plant molecular biology

authors： Li ZM,Zhang JZ,Mei L,Deng XX,Hu CG,Yao JL

更新日期：2010-09-01 00:00:00

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...

journal_title：Plant molecular biology

authors： Nechushtai R,Nelson N

更新日期：1985-11-01 00:00:00

abstract：:Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...

journal_title：Plant molecular biology

authors： Pontier D,Gan S,Amasino RM,Roby D,Lam E

更新日期：1999-04-01 00:00:00

abstract：:Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...

journal_title：Plant molecular biology

authors： Gachon C,Baltz R,Saindrenan P

更新日期：2004-01-01 00:00:00

abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

journal_title：Plant molecular biology

authors： García-Ortiz MV,Ariza RR,Roldán-Arjona T

更新日期：2001-12-01 00:00:00

abstract：:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...

journal_title：Plant molecular biology

authors： Roell MK,Morse DE

更新日期：1993-01-01 00:00:00

abstract：:Elucidating the regulatory mechanisms of plant organ formation is an important component of plant developmental biology and will be useful for crop improvement applications. Plant organ formation, or organogenesis, occurs when a group of primordial cells differentiates into an organ, through a well-orchestrated series...

journal_title：Plant molecular biology

authors： Lu XC,Gong HQ,Huang ML,Bai SL,He YB,Mao X,Geng Z,Li SG,Wei L,Yuwen JS,Xu ZH,Bai SN

更新日期：2006-08-01 00:00:00