RdDM pathway components differentially modulate Tobamovirus symptom development.

abstract：:Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. R...

journal_title：Plant molecular biology

authors： Abdelhady MI,Beuerle T,Ober D

更新日期：2009-09-01 00:00:00

abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

journal_title：Plant molecular biology

authors： Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

更新日期：2019-03-01 00:00:00

abstract：:The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

journal_title：Plant molecular biology

authors： Geffers R,Cerff R,Hehl R

更新日期：2000-05-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

journal_title：Plant molecular biology

authors： Lertpiriyapong K,Sung ZR

更新日期：2003-11-01 00:00:00

abstract：KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...

journal_title：Plant molecular biology

authors： Gong P,Luo Y,Huang F,Chen Y,Zhao C,Wu X,Li K,Yang X,Cheng F,Xiang X,Wu C,Pan G

更新日期：2019-05-01 00:00:00

abstract：:The gene coding for a new class of proteins rich in glycine and proline (GPRP) was cloned in Arabidopsis thaliana. In the protein sequence, five amino acids - glycine, proline, alanine, tyrosine and histidine - account for 79.4% of the total composition. The protein has two different glycine-rich domains interrupted b...

journal_title：Plant molecular biology

authors： Marty I,Monfort A,Stiefel V,Ludevid D,Delseny M,Puigdomènech P

更新日期：1996-02-01 00:00:00

abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...

journal_title：Plant molecular biology

authors： Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

更新日期：1990-12-01 00:00:00

abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...

journal_title：Plant molecular biology

authors： Gil P,Green PJ

更新日期：1997-07-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：:A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...

journal_title：Plant molecular biology

authors： Spence MJ,Henzl MT,Lammers PJ

更新日期：1991-09-01 00:00:00

abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...

journal_title：Plant molecular biology

authors： Kao CW,Bakshi M,Sherameti I,Dong S,Reichelt M,Oelmüller R,Yeh KW

更新日期：2016-12-01 00:00:00

abstract：:The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by anoth...

journal_title：Plant molecular biology

authors： Mikkonen A,Porali I,Cercos M,Ho TH

更新日期：1996-05-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Complex signal transduction pathways underlie the myriad plant responses to attack by pathogens. Ca(2+) is a universal second messenger in eukaryotes that modulates various signal transduction pathways through stimulus-specific changes in its intracellular concentration. Ca(2+)-binding proteins such as calmodulin (CaM...

journal_title：Plant molecular biology

authors： Chiasson D,Ekengren SK,Martin GB,Dobney SL,Snedden WA

更新日期：2005-08-01 00:00:00

abstract：:Two cDNAs encoding putative metallothionein (MT)-like peptides have been isolated from tomato (L. esculentum L.). The predicted protein products of these two cDNAs (LeMT(A) and LeMT(B)) are 72 and 83 amino acids respectively and both encode peptides with arrangements of cysteine residues characteristic of type II plan...

journal_title：Plant molecular biology

authors： Whitelaw CA,Le Huquet JA,Thurman DA,Tomsett AB

更新日期：1997-02-01 00:00:00

abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...

journal_title：Plant molecular biology

authors： Trainotti L,Spolaore S,Pavanello A,Baldan B,Casadoro G

更新日期：1999-05-01 00:00:00

abstract：:Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...

journal_title：Plant molecular biology

authors： Thanh ND,Medgyesy P

更新日期：1989-01-01 00:00:00

abstract：:Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...

journal_title：Plant molecular biology

authors： Capone I,Frugis G,Costantino P,Cardarelli M

更新日期：1994-07-01 00:00:00

abstract：:The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...

journal_title：Plant molecular biology

authors： Woessner JP,Gillham NW,Boynton JE

更新日期：1987-03-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：:A physical mapping method for unique nucleotide sequences on specific chromosomal regions was developed combining objective chromosome identification and highly sensitive fluorescence in situ hybridisation (FISH). Four unique nucleotide sequences cloned from rice genomic DNAs, varying in size from 1.3 to 400 kb, were ...

journal_title：Plant molecular biology

authors： Ohmido N,Akiyama Y,Fukui K

更新日期：1998-12-01 00:00:00

abstract：:In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

journal_title：Plant molecular biology

authors： Showalter AM,Butt AD,Kim S

更新日期：1992-05-01 00:00:00

abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...

journal_title：Plant molecular biology

authors： Miao ZH,Liu X,Lam E

更新日期：1994-04-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...

journal_title：Plant molecular biology

authors： Fukuda N,Ikawa Y,Aoyagi T,Kozaki A

更新日期：2013-07-01 00:00:00

abstract：:In this paper we present the structural analysis of the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family from Petunia hybrida. Southern blot analysis and restriction endonuclease mapping showed that two cloned regions of the petunia genome contained sequences highly homologous to a previously isolated ACC o...

journal_title：Plant molecular biology

authors： Tang X,Wang H,Brandt AS,Woodson WR

更新日期：1993-12-01 00:00:00

abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...

journal_title：Plant molecular biology

authors： Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler F

更新日期：1993-11-01 00:00:00

abstract：KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...

journal_title：Plant molecular biology

authors： Su J,Yang X,He J,Zhang Y,Duan X,Wang R,Shen W

更新日期：2019-11-01 00:00:00