Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants.

Abstract:

:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats of the A genome component. In the studies presented here, petunia plants transgenic for either both components (A×B hybrids) or the A component alone were examined for the presence of virus particles and encapsidated, single stranded viral DNA. The results of DNase protection experiments and direct observation of extracts from transgenic plants by electron microscopy indicate that single stranded TGMV DNA is in both cases packaged into paired particles identical to those obtained from virus-infected plants. DNase-treated virions isolated from A×B hybrid petunia are infectious when inoculated onto healthy Nicotiana benthamiana. Likewise, virions obtained from transgenic A petunia are infectious for plants transgenic for the B component.Our observations of TGMV replication in transgenic plants indicate that TGMV A DNA encodes all viral functions necessary for the replication and encapsidation of viral DNA. The possible role of the B component in TGMV replication is discussed.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

doi

10.1007/BF00017993

subject

Has Abstract

pub_date

1987-11-01 00:00:00

pages

477-84

issue

6

eissn

0167-4412

issn

1573-5028

journal_volume

8

pub_type

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