Abstract:
:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats of the A genome component. In the studies presented here, petunia plants transgenic for either both components (A×B hybrids) or the A component alone were examined for the presence of virus particles and encapsidated, single stranded viral DNA. The results of DNase protection experiments and direct observation of extracts from transgenic plants by electron microscopy indicate that single stranded TGMV DNA is in both cases packaged into paired particles identical to those obtained from virus-infected plants. DNase-treated virions isolated from A×B hybrid petunia are infectious when inoculated onto healthy Nicotiana benthamiana. Likewise, virions obtained from transgenic A petunia are infectious for plants transgenic for the B component.Our observations of TGMV replication in transgenic plants indicate that TGMV A DNA encodes all viral functions necessary for the replication and encapsidation of viral DNA. The possible role of the B component in TGMV replication is discussed.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DMdoi
10.1007/BF00017993subject
Has Abstractpub_date
1987-11-01 00:00:00pages
477-84issue
6eissn
0167-4412issn
1573-5028journal_volume
8pub_type
杂志文章abstract::The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005874404706
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028854
更新日期:1994-11-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1995-12-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2003-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039423
更新日期:1990-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020226
更新日期:1995-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006081009173
更新日期:1998-11-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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更新日期:2005-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00041405
更新日期:1996-12-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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更新日期:2013-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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更新日期:2009-05-01 00:00:00