Subtractive cDNA libraries identify differentially expressed genes in dormant and growing buds of leafy spurge (Euphorbia esula).

Abstract:

:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A total of 516 unique sequences were obtained from 2304 minimally redundant clones. Radioactive probes developed from RNAs extracted from crown buds of either intact (para-dormant control) or a series of growth-induced (2 h, 2, and 4 d after decapitation) plants were used to identify differentially expressed genes by macroarray analysis. Semi-quantitative RT-PCR was used to confirm results obtained by macroarray analysis and to determine the expression profiles for other transcripts identified within the subtractive libraries. Selected clones were also used to examine gene expression in crown buds after growth induction and/or during normal seasonal growth. In this study, four distinct patterns of gene expression were observed during the transition from para-dormancy to growth-induction. Many of the differentially regulated genes identified have unknown or hypothetical functions while others are known to play important roles in molecular functions. Gene ontology analysis identified a greater proportion of genes involved with catalytic activity in the forward library while the reverse library had a greater proportion of genes involved in DNA/RNA binding.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

doi

10.1007/s11103-006-0015-x

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

329-44

issue

1-2

eissn

0167-4412

issn

1573-5028

journal_volume

61

pub_type

杂志文章
  • Hormone interactions during vascular development.

    abstract::Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1007/s11103-008-9374-9

    authors: Dettmer J,Elo A,Helariutta Y

    更新日期:2009-03-01 00:00:00

  • Genome-wide identification of housekeeping genes in maize.

    abstract::In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0246-1

    authors: Lin F,Jiang L,Liu Y,Lv Y,Dai H,Zhao H

    更新日期:2014-11-01 00:00:00

  • Mesophyll-specific, light and metabolic regulation of the C4 PPCZm1 promoter in transgenic maize.

    abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006487326533

    authors: Kausch AP,Owen TP Jr,Zachwieja SJ,Flynn AR,Sheen J

    更新日期:2001-01-01 00:00:00

  • Temporal and tissue-specific expression of the tobacco ntf4 MAP kinase.

    abstract::The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1010645431133

    authors: Voronin V,Touraev A,Kieft H,van Lammeren AA,Heberle-Bors E,Wilson C

    更新日期:2001-04-01 00:00:00

  • Gene expression of nitrite reductase in Scots pine (Pinus sylvestris L.) as affected by light and nitrate.

    abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00043873

    authors: Neininger A,Seith B,Hoch B,Mohr H

    更新日期:1994-06-01 00:00:00

  • Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element.

    abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005780529457

    authors: Grimmig B,Matern U

    更新日期:1997-01-01 00:00:00

  • Modification of reactive oxygen species scavenging capacity of chloroplasts through plastid transformation.

    abstract::Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals are generated through normal biochemical processes, but their production is increased by abiotic stresses. The prospects for enhancing ROS scavenging, and hence stress tolerance, by direct gene expression in a vulnerabl...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9784-y

    authors: Poage M,Le Martret B,Jansen MA,Nugent GD,Dix PJ

    更新日期:2011-07-01 00:00:00

  • Isolation, characterization and expression of the maize Cat2 catalase gene.

    abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020803

    authors: Guan L,Polidoros AN,Scandalios JG

    更新日期:1996-03-01 00:00:00

  • Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogen

    abstract::A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005915507497

    authors: Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

    更新日期:1998-02-01 00:00:00

  • Profiling methyl jasmonate-responsive transcriptome for understanding induced systemic resistance in whitebark pine (Pinus albicaulis).

    abstract:KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-017-0655-z

    authors: Liu JJ,Williams H,Li XR,Schoettle AW,Sniezko RA,Murray M,Zamany A,Roke G,Chen H

    更新日期:2017-11-01 00:00:00

  • Nucleotide sequence of psbC, the gene encoding the CP-43 chlorophyll a-binding protein of Photosystem II, in the cyanobacterium Synechocystis 6803.

    abstract::The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00029879

    authors: Chisholm D,Williams JG

    更新日期:1988-07-01 00:00:00

  • IMPERFECTIVE EXINE FORMATION (IEF) is required for exine formation and male fertility in Arabidopsis.

    abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01114-8

    authors: Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

    更新日期:2021-01-22 00:00:00

  • The structure of a Phaseolus vulgaris cDNA encoding the iron storage protein ferritin.

    abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040644

    authors: Spence MJ,Henzl MT,Lammers PJ

    更新日期:1991-09-01 00:00:00

  • Acidic and basic class III chitinase mRNA accumulation in response to TMV infection of tobacco.

    abstract::Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00027070

    authors: Lawton K,Ward E,Payne G,Moyer M,Ryals J

    更新日期:1992-08-01 00:00:00

  • Small grain and semi-dwarf 3, a WRKY transcription factor, negatively regulates plant height and grain size by stabilizing SLR1 expression in rice.

    abstract:KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01049-0

    authors: Lan J,Lin Q,Zhou C,Ren Y,Liu X,Miao R,Jing R,Mou C,Nguyen T,Zhu X,Wang Q,Zhang X,Guo X,Liu S,Jiang L,Wan J

    更新日期:2020-11-01 00:00:00

  • High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda).

    abstract::Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006126609534

    authors: Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

    更新日期:1999-02-01 00:00:00

  • Cell cycle regulation of the microtubular cytoskeleton.

    abstract::The microtubular element of the plant cytoskeleton undergoes dramatic architectural changes in the course of the cell cycle, specifically at the entry into and exit from mitosis. These changes underlie the acquisition of specialized properties and functions involved, for example, in the equal segregation of chromosome...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1006346107807

    authors: Vantard M,Cowling R,Delichère C

    更新日期:2000-08-01 00:00:00

  • Computational prediction and experimental verification of HVA1-like abscisic acid responsive promoters in rice (Oryza sativa).

    abstract::Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9017-y

    authors: Ross C,Shen QJ

    更新日期:2006-09-01 00:00:00

  • Coordinate modulation of maize sulfate permease and ATP sulfurylase mRNAs in response to variations in sulfur nutritional status: stereospecific down-regulation by L-cysteine.

    abstract::To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006148815106

    authors: Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

    更新日期:1999-02-01 00:00:00

  • Post-transcriptional control of cell type-specific gene expression in bundle sheath and mesophyll chloroplasts of Amaranthus hypochondriacus.

    abstract::Plants that utilize the highly efficient C4 photosynthetic pathway possess two types of specialized leaf cells, the mesophyll and bundle sheath. In mature leaves of amaranth, a dicotyledonous C4 plant, ribulose 1,5-bisphosphate carboxylase (Rubisco) is localized specifically to the chloroplasts of bundle sheath cells,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00015971

    authors: Boinski JJ,Wang JL,Xu P,Hotchkiss T,Berry JO

    更新日期:1993-06-01 00:00:00

  • A maize histone deacetylase and retinoblastoma-related protein physically interact and cooperate in repressing gene transcription.

    abstract::In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1022090916446

    authors: Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

    更新日期:2003-02-01 00:00:00

  • Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum.

    abstract::Type I signal peptidases are a widespread family of enzymes which remove the presequences from proteins translocated across cell membranes, including thylakoid and cytoplasmic membranes of cyanobacteria and thylakoid membranes of chloroplasts. We have cloned and sequenced a signal peptidase gene from the thermophilic ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019191

    authors: Packer JC,André D,Howe CJ

    更新日期:1995-01-01 00:00:00

  • Precursor of the nuclear-encoded extrinsic 30 kDa protein in photosystem II of Euglena gracilis Z is not a polyprotein.

    abstract::Polyprotein-type precursors have been reported for the nuclear-encoded proteins such as the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the apoproteins of light-harvesting chlorophyll-protein (LHC) in Euglena. We report here that the precursor of the extrinsic 30 kDa protein of photo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00039627

    authors: Kuroda I,Inagaki J,Yamamoto Y

    更新日期:1993-01-01 00:00:00

  • Regulatory activity exerted by the SAUR-AC1 promoter region in transgenic plants.

    abstract::SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005875300606

    authors: Gil P,Green PJ

    更新日期:1997-07-01 00:00:00

  • Identification and characterization of (GA/CT)n-microsatellite loci from Quercus petraea.

    abstract::In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005736722794

    authors: Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

    更新日期:1997-04-01 00:00:00

  • Auxin-induced mRNA species in tobacco cell cultures.

    abstract::Using a 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell-suspension culture we analyzed early hormone-induced molecular events preceding cell division. By differential screening of a cDNA library to mRNAs derived from hormone-starved cells treated with 2,4-D for 4 h, seven non-cross hybridizing cDNA clone...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00016152

    authors: van der Zaal EJ,Memelink J,Mennes AM,Quint A,Libbenga KR

    更新日期:1987-03-01 00:00:00

  • Expression analysis of a sucrose carrier in the germinating seedling of Ricinus communis.

    abstract::This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006040306581

    authors: Bick JA,Neelam A,Smith E,Nelson SJ,Hall JL,Williams LE

    更新日期:1998-10-01 00:00:00

  • CYCLIN-DEPENDENT KINASE G2 regulates salinity stress response and salt mediated flowering in Arabidopsis thaliana.

    abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0324-z

    authors: Ma X,Qiao Z,Chen D,Yang W,Zhou R,Zhang W,Wang M

    更新日期:2015-06-01 00:00:00

  • The structure of the large spacer region of the rDNA in Vicia faba and Pisum sativum.

    abstract::The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019395

    authors: Kato A,Nakajima T,Yamashita J,Yakura K,Tanifuji S

    更新日期:1990-06-01 00:00:00

  • Transcriptional and metabolic profiles of stress-induced, embryogenic tobacco microspores.

    abstract::Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9078-y

    authors: Hosp J,Tashpulatov A,Roessner U,Barsova E,Katholnigg H,Steinborn R,Melikant B,Lukyanov S,Heberle-Bors E,Touraev A

    更新日期:2007-01-01 00:00:00